Electroacupuncture inhibits apoptosis of splenic lymphocytes in traumatized rats through modulation of the TNF-α/NF-κB signaling pathway
Electroacupuncture inhibits apoptosis of splenic lymphocytes in traumatized rats through modulation of the TNF-α/NF-κB signaling pathway
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DOI：
10.3892/mmr.2014.2740
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发表时间：
2015-01-01
影响因子：
3.4
通讯作者：
Li, Hulun
中科院分区：
文献类型：
--
作者：
Wang, Kun;Wu, Huaxing;Li, Hulun
手术创伤会导致免疫系统的严重恶化。电针（EA）可以改善手术后发生的免疫缺陷，但是，潜在的信号机制需要进一步研究。在本研究中，将40只大鼠随机分为四组：对照组;对照+电针组;创伤组;创伤+电针组。术后即刻电针足三里、兰卫穴。术后24 h分离大鼠脾脏T细胞。流式细胞仪检测淋巴细胞凋亡率，免疫印迹法检测caspase-3、caspase-8、肿瘤坏死因子（TNF）-α和TNF受体1（TNFR 1）蛋白表达水平。使用基于Trans-AM（（R））ELISA的试剂盒测定核因子（NF）-B的DNA结合活性。本研究结果表明，手术创伤诱导脾淋巴细胞凋亡，并显著增加caspase-3和caspase-8的蛋白表达水平。这是伴随着TNF-α和TNFR 1的表达水平的增加，并在脾T细胞中的NF-B的活性显着降低。电针可显著降低caspase-3、caspase-8、TNF-α和TNFR-1的表达水平，提高NF-B活性，抑制淋巴细胞凋亡率。提示电针可抑制手术创伤诱导的脾淋巴细胞凋亡，改善术后免疫抑制。这可能是通过下调TNF-α表达水平和上调NF-B活性介导的。
Surgical trauma leads to a severe deterioration of the immune system. Electroacupuncture (EA) may improve the immunodeficiency that occurs following surgery; however, the underlying signaling mechanisms require further study. In the present study, 40 rats were equally randomized into four groups: Control; Control + EA; Trauma; Trauma + EA. EA was applied at the Zusanli' (ST36) and Lanwei' (Extra37) acupoints, immediately following surgery. The splenic T cells were isolated from the rats 24 h after surgery. The apoptotic rate of the lymphocytes was measured by flow cytometric analysis, and western blotting was used to determine the protein expression levels of caspase-3, caspase-8, tumor necrosis factor (TNF)- and TNF receptor 1 (TNFR1). The DNA binding activity of nuclear factor (NF)-B was determined using Trans-AM((R)) ELISA-based kits. The results of the present study showed that surgical trauma induced apoptosis of splenic lymphocytes, and significantly increased the protein expression levels of caspase-3 and caspase-8. This was accompanied by increased expression levels of TNF- and TNFR1, and a marked reduction in the activity of NF-B in splenic T cells. Administration of EA significantly decreased the expression levels of caspase-3, caspase-8, TNF- and TNFR1, elevated the activity of NF-B, and suppressed the apoptotic rate of the lymphocytes. The data suggests that EA may inhibit the apoptosis of splenic lymphocytes induced by surgical trauma, and ameliorate the postoperative immunosuppression. This may be mediated by the downregulation of TNF- expression levels and upregulation of the activity of NF-B.