2D or 3D? How cell motility measurements are conserved across dimensions in vitro and translate in vivo
2D or 3D? How cell motility measurements are conserved across dimensions in vitro and translate in vivo
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DOI：
10.1002/btm2.10148
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发表时间：
2019-12-09
影响因子：
7.4
通讯作者：
Munson, Jennifer M.
中科院分区：
文献类型：
--
作者：
Galarza, Sualyneth;Kim, Hyuna;Munson, Jennifer M.
细胞运动是几个过程的关键方面，如伤口愈合和免疫；然而，它在癌症中是失调的。目前的成像工具的限制使得很难研究细胞在体内的迁移。为了克服这一点，并从调节细胞迁移的微环境中识别驱动因素，生物工程师开发了2D（二维）和3D（三维）组织模型系统，用于研究体外细胞运动，目的是模仿细胞在体内移动的环境元素。然而，目前还没有系统的研究明确地联系和比较这些几何形状或系统之间的细胞运动测量。在这里，我们对我们自己的数据以及现有文献中的数据进行了这样的分析，以了解度量是否以及哪些度量是跨系统保守的。令我们惊讶的是，只有一项2D表面上的细胞运动指标与3D环境中的细胞迁移显著正相关（迁移细胞百分比），而3D环境中的细胞侵袭与体内胶质母细胞瘤侵袭呈弱负相关。最后，为了比较复杂的模型系统、体内数据和来自不同实验室的数据，我们建议在进行影响细胞运动的实验时，研究小组报告一个效应量，这是一个在实验和实验室中最可翻译的统计工具。
Cell motility is a critical aspect of several processes, such as wound healing and immunity; however, it is dysregulated in cancer. Current limitations of imaging tools make it difficult to study cell migration in vivo. To overcome this, and to identify drivers from the microenvironment that regulate cell migration, bioengineers have developed 2D (two-dimensional) and 3D (three-dimensional) tissue model systems in which to study cell motility in vitro, with the aim of mimicking elements of the environments in which cells move in vivo. However, there has been no systematic study to explicitly relate and compare cell motility measurements between these geometries or systems. Here, we provide such analysis on our own data, as well as across data in existing literature to understand whether, and which, metrics are conserved across systems. To our surprise, only one metric of cell movement on 2D surfaces significantly and positively correlates with cell migration in 3D environments (percent migrating cells), and cell invasion in 3D has a weak, negative correlation with glioblastoma invasion in vivo. Finally, to compare across complex model systems, in vivo data, and data from different labs, we suggest that groups report an effect size, a statistical tool that is most translatable across experiments and labs, when conducting experiments that affect cellular motility.