Expression of Streptococcus pneumoniae antigens, PsaA (pneumococcal surface antigen A) and PspA (pneumococcal surface protein A) by Lactobacillus casei
Expression of Streptococcus pneumoniae antigens, PsaA (pneumococcal surface antigen A) and PspA (pneumococcal surface protein A) by Lactobacillus casei
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DOI：
10.1016/s0378-1097(03)00645-1
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发表时间：
2003-10-10
影响因子：
2.1
通讯作者：
Pérez-Martínez, G
中科院分区：
文献类型：
--
作者：
Oliveira, MLS;Monedero, V;Pérez-Martínez, G
最近的许多乳酸菌研究工作旨在设计新菌株，这些菌株可用作活载体，用于递送口服疫苗或其他治疗分子的抗原。在这项工作中，基于干酪乳杆菌乳糖操纵子启动子的诱导表达系统用于在该乳酸菌中表达肺炎链球菌的三种重要表面抗原：毒力相关肺炎球菌表面抗原（PsaA）和毒力因子PspA（肺炎球菌表面蛋白A）的两种变体。这三种蛋白质的表达在乳糖上生长时被诱导，并被葡萄糖强烈抑制。这些蛋白质是在细胞内产生的。此外，通过与干酪乳杆菌表面蛋白酶的分泌和处理信号融合，实现向生长培养基的分泌。有趣的是，在培养物上清液中发现了分泌的 PspA 蛋白。 PsaA 仍然被困在细胞壁中。肺炎球菌抗原在食品级生物体中的表达为针对这种重要病原体的粘膜疫苗接种开辟了另一种选择。 (C) 2003 年欧洲微生物学会联合会。由 Elsevier B.V. 出版。保留所有权利。
A number of recent research works in lactic acid bacteria aim towards the design of new strains that could be used as live vectors for the delivery of antigens for oral vaccination, or other therapeutic molecules. In this work, an inducible expression system based on the Lactobacillus casei lactose operon promoter was used to express three important surface antigens of Streptococcus pneumoniae in this lactic acid bacterium: a virulence-related pneumococcal surface antigen (PsaA) and two variants of the virulence factor PspA (pneumococcal surface protein A). Expression of the three proteins was induced upon growth on lactose and strongly repressed by glucose. These proteins were produced intracellularly. Also, secretion to the growth medium was achieved by means of a fusion to the secreting and processing signals from the L. casei surface proteinase. Interestingly, while secreted PspA proteins were found in the culture supernatants. PsaA remained trapped in the cell wall. Expression of pneumococcal antigens in a food-grade organism opens an alternative for mucosal vaccination against this important pathogen. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.