Challenges and solutions for the identification of membrane proteins in non-model plants.
Challenges and solutions for the identification of membrane proteins in non-model plants.
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DOI：
10.1016/j.jprot.2011.02.016
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发表时间：
2011-08
影响因子：
3.3
通讯作者：
A. Vertommen;B. Panis;R. Swennen;R. Swennen;S. Carpentier
中科院分区：
文献类型：
--
作者：
A. Vertommen;B. Panis;R. Swennen;R. Swennen;S. Carpentier
非模式植物蛋白质组学的主力是经典的双向电泳法，即等电聚焦和十二烷基硫酸钠-PAGE的结合。然而，具有多个跨膜结构域的膜蛋白在经典的双向凝胶上很难检测到，因为它们的丰度很低，在水介质中的溶解性很差。在目前的综述中，讨论了在非模型工厂中解决这两个问题的解决方案。概述了为膜蛋白质组学开发的替代技术，并对其优缺点进行了比较。随后，讨论了评价膜蛋白鉴定的不同技术和方法的优缺点。最后，对最近的植物膜蛋白质组研究进行了概述，并列出了所使用的分离技术和已鉴定的膜蛋白的数量。
The workhorse for proteomics in non-model plants is classical two-dimensional electrophoresis, a combination of iso-electric focusing and SDS-PAGE. However, membrane proteins with multiple membrane spanning domains are hardly detected on classical 2-DE gels because of their low abundance and poor solubility in aqueous media. In the current review, solutions that have been proposed to handle these two problems in non-model plants are discussed. An overview of alternative techniques developed for membrane proteomics is provided together with a comparison of their strong and weak points. Subsequently, strengths and weaknesses of the different techniques and methods to evaluate the identification of membrane proteins are discussed. Finally, an overview of recent plant membrane proteome studies is provided with the used separation technique and the number of identified membrane proteins listed.