Quaternized chitosan-Matrigel-polyacrylamide hydrogels as wound dressing for wound repair and regeneration
Quaternized chitosan-Matrigel-polyacrylamide hydrogels as wound dressing for wound repair and regeneration
复制标题
季铵化壳聚糖-基质胶-聚丙烯酰胺水凝胶作为伤口敷料用于伤口修复和再生。
DOI：
10.1016/j.carbpol.2019.115302
复制
发表时间：
2019-12-15
影响因子：
11.2
通讯作者：
Liu, Guohui
中科院分区：
文献类型：
--
作者：
Xue, Hang;Hu, Liangcong;Liu, Guohui
水凝胶可能是一种很有前途的伤口愈合敷料，它可以维持伤口部位的湿润环境并加速伤口愈合。然而，缺乏抗菌作用、合适的机械性能和粘合性限制了它们的应用。在这里，我们设计了一种具有多功能的季铵化壳聚糖-基质胶-聚丙烯酰胺（QCS-M-PAM）水凝胶。对杂化水凝胶的形态、溶胀率、力学测试、抗菌性能、止血性能和生物相容性进行了体外和体内研究。该杂化水凝胶具有三维（3D）微孔结构、高溶胀比、优异的拉伸和压缩性能、与人体皮肤相似的模量、良好的粘附性和低细胞毒性。体内组织学和分子测试结果表明，混合水凝胶可以通过上调抗炎因子和下调促炎因子来显着促进伤口愈合、胶原沉积并诱导皮肤附件再生。总之，目前具有止血和粘合性能的抗菌水凝胶被认为具有用作全层皮肤缺损伤口敷料的广阔潜力。
Hydrogels could be promising wound healing dressings that maintain a moist environment in the wound site and accelerate wound healing. However, the lack of antibacterial effect, suitable mechanical property and adhesiveness limits their applications. Here, we designed a quaternized chitosan-Matrigel-polyacrylamide (QCS-M-PAM) hydrogel with multi-functions. The morphology, swelling ratio, mechanical test, antimicrobial property, hemostatic performance and biocompatibility of the hybrid hydrogel were investigated in vitro and vivo. The hybrid hydrogel showed a three-dimensional (3D) microporous structure, high swelling ratio, excellent stretchable and compressive property, similar modulus to human skin, good adhesiveness, and low cytotoxicity. The results of histology and molecular testing in vivo demonstrated that the hybrid hydrogel could significantly enhance wound healing, collagen deposition, and induce skin adnexal regeneration by upregulating anti-inflammatory factors, and downregulating proinflammatory factors. Together, the present antibacterial hydrogels with hemostatic and adhesive properties are considered to have promising potential used as wound dressings for full-thickness skin defect.