Drosophila melanogaster: A model to study obesity effects on genes expression and developmental changes on descendants
Drosophila melanogaster: A model to study obesity effects on genes expression and developmental changes on descendants
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DOI：
10.1002/jcb.26724
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发表时间：
2018-07-01
影响因子：
4
通讯作者：
Prigol, Marina
中科院分区：
文献类型：
--
作者：
de Paula, Mariane T.;Silva, Marcia R. P.;Prigol, Marina
母亲肥胖和代谢性疾病是对后代最重要的潜在危险，因为受损的后代可能导致损害成年生活和健康的缺陷。本研究主要探讨了氧化损伤、酶抗氧化防御以及脂肪酸代谢相关酶如酰基辅酶A合成酶和乙酰辅酶A合成酶的活性（mRNA表达水平），以及调节细胞应激信号传导途径，如Hsp83，在接受高脂饮食（HFD）的果蝇模型中的基因表达和胰岛素样肽DILP 6（10%和20%的椰子油）在整个开发期间。7天后，取出祖蝇，每天监测剩余的卵，直到羽化。然后将后代暴露于常规饮食（RD）。结果表明，HFD导致羽化比例，寿命，线粒体富集组分中MTT减少，AceCS1水平，mRNA表达水平（SOD和CAT）降低，并且仅在接受最高浓度椰子油的组中观察到过氧化氢酶活性降低。同时，证明了HSP 83 mRNA水平的上调增加，但仅当加入10%的椰子油时，葡萄糖和甘油三酯水平增加，以及在更大浓度的椰子油（20%）中DILP 6 mRNA水平增加。总之，具有用HFD喂养的祖细胞的果蝇可以发展代谢功能障碍，引起氧化损伤，这与寿命缩短有关。
Maternal obesity and metabolic diseases are two of the most important potential dangers to offspring, given that impaired offspring may cause deficiencies that impair the adult life and health. This study evaluated the oxidative damage, the enzymatic antioxidant defenses, and the enzymes of fatty acid metabolism, such as Acyl-CoA Synthetase and Acetyl-CoA Synthetase (mRNA expression levels), as well as the modulation of cell stress signaling pathway, as Hsp83, and gene expression and insulin-like peptide DILP6 in Drosophila melanogaster models that received a high fat diet (HFD) (10% and 20% of coconut oil) throughout their development period. After 7 days, the progenitor flies were removed and, the remaining eggs were monitored daily, until the eclosion. The descendants were then exposed to a regular diet (RD). The results revealed that the HFD caused a decrease in the proportion of eclosion, lifespan, MTT reduction in mitochondrial enriched fractions, AceCS1 levels, mRNA expression levels (SOD and CAT), and in catalase activity a decrease was only observed in the group that received the highest concentration of coconut oil. In parallel, it was demonstrated an increase in the upregulation of HSP83 mRNA levels, but only when 10% of coconut oil was added, and an increase in glucose and triglyceride levels, as well as in DILP6 mRNA levels in larger concentration of coconut oil tested (20%). In conclusion, flies that have progenitors fed with HFD can develop metabolic dysfunctions, causing oxidative insults, which are involved in the shortening of lifespan.