Antigen Production in Plant to Tackle Infectious Diseases Flare Up: The Case of SARS.
Antigen Production in Plant to Tackle Infectious Diseases Flare Up: The Case of SARS.
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DOI：
10.3389/fpls.2016.00054
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发表时间：
2016
影响因子：
5.6
通讯作者：
Franconi R
中科院分区：
文献类型：
--
作者：
Demurtas OC;Massa S;Illiano E;De Martinis D;Chan PK;Di Bonito P;Franconi R
严重急性呼吸综合征(SARS)是一种具有大流行潜力的危险感染。它于2002年出现，其病原体SARS冠状病毒(SARS-CoV)跨越物种障碍感染人类，表现出较高的发病率和死亡率。目前还没有为SARS冠状病毒颁发疫苗许可证，在第一次暴发期间为开发诊断工具做出了重要努力。在这里，我们证明了SARS冠状病毒的两个重要的抗原决定簇，核衣壳蛋白(N)和膜蛋白(M)分别利用病毒衍生载体或农业渗透在本氏烟草中瞬时表达。对于M蛋白，这是第一次描述植物中产生的N蛋白，而对于植物来源的N蛋白，我们证明它被2003年香港SARS疫情患者的血清识别。来自植物的重组N和M蛋白的可获得性为进一步评估它们对开发诊断和保护/治疗工具的潜在效用打开了道路，这些诊断和保护/治疗工具将以低成本和最小的风险快速制造，以应对潜在的新的高传染性SARS-CoV疫情。
Severe acute respiratory syndrome (SARS) is a dangerous infection with pandemic potential. It emerged in 2002 and its aetiological agent, the SARS Coronavirus (SARS-CoV), crossed the species barrier to infect humans, showing high morbidity and mortality rates. No vaccines are currently licensed for SARS-CoV and important efforts have been performed during the first outbreak to develop diagnostic tools. Here we demonstrate the transient expression in Nicotiana benthamiana of two important antigenic determinants of the SARS-CoV, the nucleocapsid protein (N) and the membrane protein (M) using a virus-derived vector or agro-infiltration, respectively. For the M protein, this is the first description of production in plants, while for plant-derived N protein we demonstrate that it is recognized by sera of patients from the SARS outbreak in Hong Kong in 2003. The availability of recombinant N and M proteins from plants opens the way to further evaluation of their potential utility for the development of diagnostic and protection/therapy tools to be quickly manufactured, at low cost and with minimal risk, to face potential new highly infectious SARS-CoV outbreaks.