Gas Vesicle Nanoparticles for Antigen Display.
Gas Vesicle Nanoparticles for Antigen Display.
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DOI：
10.3390/vaccines3030686
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发表时间：
2015-09-07
期刊：
影响因子：
7.8
通讯作者：
DasSarma P
中科院分区：
文献类型：
--
作者：
DasSarma S;DasSarma P
微生物如嗜盐古菌Halobacterium sp. NRC-1产生充满气体的漂浮细胞器，这些细胞器很容易被纯化为蛋白质纳米颗粒（称为气泡或GVNPs）。GVNP是无毒的、异常稳定的、生物工程化的和自我辅助的。一个编码十几种蛋白质的大型基因簇与它们的生物起源有关。在纳米颗粒的外表面上发现的一种蛋白质GvpC可以容纳C末端区域附近的插入，并导致GVNPs在纳米颗粒的表面上显示插入的序列。在这里，我们回顾了GVNP的结构和生物发生的知识，以及现有的研究，致病性病毒，细菌和真核生物的蛋白质和肽的纳米粒子上显示的免疫原性。讨论了GVNPs生物工程的遗传工具的最新进展，沿着讨论了疫苗和其他应用开发的未来机遇和挑战。
Microorganisms like the halophilic archaeon Halobacterium sp. NRC-1 produce gas-filled buoyant organelles, which are easily purified as protein nanoparticles (called gas vesicles or GVNPs). GVNPs are non-toxic, exceptionally stable, bioengineerable, and self-adjuvanting. A large gene cluster encoding more than a dozen proteins has been implicated in their biogenesis. One protein, GvpC, found on the exterior surface of the nanoparticles, can accommodate insertions near the C-terminal region and results in GVNPs displaying the inserted sequences on the surface of the nanoparticles. Here, we review the current state of knowledge on GVNP structure and biogenesis as well as available studies on immunogenicity of pathogenic viral, bacterial, and eukaryotic proteins and peptides displayed on the nanoparticles. Recent improvements in genetic tools for bioengineering of GVNPs are discussed, along with future opportunities and challenges for development of vaccines and other applications.