Oomycete small RNAs bind to the plant RNA-induced silencing complex for virulence
Oomycete small RNAs bind to the plant RNA-induced silencing complex for virulence
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DOI：
10.7554/elife.56096
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发表时间：
2020-05-22
期刊：
影响因子：
7.7
通讯作者：
Weiberg, Arne
中科院分区：
文献类型：
--
作者：
Dunker, Florian;Trutzenberg, Adriana;Weiberg, Arne
宿主和病原体之间的小rna （sRNAs）交换可导致受体生物中的基因沉默，这种机制被称为跨界RNAi （ck-RNAi）。虽然已经建立了促进毒力的真菌sRNAs，但ck-RNAi在不同植物病原体中的意义尚不清楚。在这里，我们描述了病原拟南芥透明operonospora arabidsidis的sRNAs，它代表了卵菌界，在系统发育上与真菌相距遥远，利用宿主植物的Argonaute (AGO)/ rna诱导的沉默复合体来产生毒力。为了证明拟南芥sRNA （HpasRNA）在时钟- rnai中的功能，我们设计了一种新的CRISPR核糖核酸内切酶Csy4/GUS报告基因，能够原位可视化HpasRNA诱导的拟南芥靶标抑制。HpasRNAs和AtAGO1在植物阿塔戈突变体和转基因拟南芥中表达的短串联靶标模拟物阻断HpasRNAs在毒力中的重要作用被揭示，两者都表现出增强的抗性。hpasrna靶向植物基因有助于宿主免疫，因为拟南芥基因敲除突变体在数量上表现出增强的易感性。
The exchange of small RNAs (sRNAs) between hosts and pathogens can lead to gene silencing in the recipient organism, a mechanism termed cross-kingdom RNAi (ck-RNAi). While fungal sRNAs promoting virulence are established, the significance of ck-RNAi in distinct plant pathogens is not clear. Here, we describe that sRNAs of the pathogen Hyaloperonospora arabidopsidis, which represents the kingdom of oomycetes and is phylogenetically distant from fungi, employ the host plant's Argonaute (AGO)/RNA-induced silencing complex for virulence. To demonstrate H. arabidopsidis sRNA (HpasRNA) functionality in ck-RNAi, we designed a novel CRISPR endoribonuclease Csy4/GUS reporter that enabled in situ visualization of HpasRNA-induced target suppression in Arabidopsis. The significant role of HpasRNAs together with AtAGO1 in virulence was revealed in plant atagol mutants and by transgenic Arabidopsis expressing a short-tandem-target-mimic to block HpasRNAs, that both exhibited enhanced resistance. HpasRNA-targeted plant genes contributed to host immunity, as Arabidopsis gene knockout mutants displayed quantitatively enhanced susceptibility.