Arabidopsis ABI5 subfamily members have distinct DNA-binding and transcriptional activities
Arabidopsis ABI5 subfamily members have distinct DNA-binding and transcriptional activities
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DOI：
10.1104/pp.003566
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发表时间：
2002-10-01
期刊：
影响因子：
7.4
通讯作者：
Thomas, TL
中科院分区：
文献类型：
--
作者：
Kim, SY;Ma, JZ;Thomas, TL
一个新的碱性亮氨酸拉链蛋白的小家族，包括脱落酸（阿坝）-不敏感5（ABI 5）结合到莱亚类基因Dc 3的启动子区。这些因子被称为AtDPBFs（拟南芥DO启动子结合因子），是从未成熟种子cDNA文库中分离的。AtDPBF与Dc 3启动子中的胚胎特化和ABA响应元件结合，并且是独特的，因为它们可以与不包含大多数其他植物碱性亮氨酸拉链蛋白结合所需的ACCT核心序列的顺式元件相互作用。全长cDNA的分析表明，至少有五种不同的Dc 3启动子结合因子存在于拟南芥种子，其中之一，AtDPBF-1，是相同的ABI 5。AtDPBF-1/AB 15 mRNA在幼苗中可被外源阿坝诱导。尽管AtDPBF的基本结构域几乎相同，但其DNA结合、二聚化和转录活性不同。
A small family of novel basic leucine zipper proteins that includes abscisic acid (ABA)-INSENSITIVE 5 (ABI5) binds to the promoter region of the lea class gene Dc3. The factors, referred to as AtDPBFs (Arabidopsis DO promoter-binding factors), were isolated from an immature seed cDNA library. AtDPBFs bind to the embryo specification and ABA-responsive elements in the Dc3 promoter and are unique in that they can interact with cis-elements that do not contain the ACCT core sequence required for the binding of most other plant basic leucine zipper proteins. Analysis of full-length cDNAs showed that at least five different Dc3 promoter-binding factors are present in Arabidopsis seeds; one of these, AtDPBF-1, is identical to ABI5. As expected, AtDPBF-1/ABl5 mRNA is inducible by exogenous ABA in seedlings. Despite the near identity in their basic domains, AtDPBFs are distinct in their DNA-binding, dimerization, and transcriptional activity.