Differentiating salmonid migratory ecotypes through stable isotope analysis of collagen: Archaeological and ecological applications
Differentiating salmonid migratory ecotypes through stable isotope analysis of collagen: Archaeological and ecological applications
复制标题
DOI：
10.1371/journal.pone.0232180
复制
发表时间：
2020-04-28
期刊：
影响因子：
3.7
通讯作者：
Szpak, Paul
中科院分区：
文献类型：
--
作者：
Guiry, Eric;Royle, Thomas C. A.;Szpak, Paul
区分不同迁移行为的能力（例如，在鱼类中的浮游生物学（anadromy和potamodromy）可以为许多水生物种的生态学、进化和保护提供重要的见解。我们提出了一个简单的稳定的碳同位素（三角洲C-13）的方法来区分sockeye（溯河产卵的海洋移民）和kokanee（potamodromous淡水居民），两个迁移生态类型的Oncorhynchus nerka（鲑科），适用于整个北太平洋沿海地区的大部分范围。kokanee（n = 239）和sockeye（n = 417）从87个网站跨越北太平洋（俄罗斯到加州）的分析表明，溯河产卵和potamodromous生态型的三角洲C-13值的基础上，他们的规模和骨胶原蛋白的广泛区分。我们提出了三个案例研究，展示了这种方法如何解决考古学，档案和保护研究中的问题。相对于确定迁徙状态的传统方法（通常对耳石进行化学分析或涉及组织的遗传分析），这里概述的d13 C方法具有非致命（应用于鳞片时）、具有成本效益、广泛使用的优点。商业化，并且应该更广泛地用于解决考古问题，因为在考古遗址发现耳石的情况很少见。
The ability to distinguish between different migratory behaviours (e.g., anadromy and potamodromy) in fish can provide important insights into the ecology, evolution, and conservation of many aquatic species. We present a simple stable carbon isotope (delta C-13) approach for distinguishing between sockeye (anadromous ocean migrants) and kokanee (potamodromous freshwater residents), two migratory ecotypes of Oncorhynchus nerka (Salmonidae) that is applicable throughout most of their range across coastal regions of the North Pacific Ocean. Analyses of kokanee (n = 239) and sockeye (n = 417) from 87 sites spanning the North Pacific (Russia to California) show that anadromous and potamodromous ecotypes are broadly distinguishable on the basis of the delta C-13 values of their scale and bone collagen. We present three case studies demonstrating how this approach can address questions in archaeology, archival, and conservation research. Relative to conventional methods for determining migratory status, which typically apply chemical analyses to otoliths or involve genetic analyses of tissues, the d13C approach outlined here has the benefit of being non-lethal (when applied to scales), cost-effective, widely available commercially, and should be much more broadly accessible for addressing archaeological questions since the recovery of otoliths at archaeological sites is rare.