Expression of the zinc finger gene fez-like in zebrafish forebrain
Expression of the zinc finger gene fez-like in zebrafish forebrain
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DOI：
10.1016/s0925-4773(00)00418-4
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发表时间：
2000-10-01
影响因子：
2.6
通讯作者：
Hibi, M
中科院分区：
文献类型：
--
作者：
Hashimoto, H;Yabe, T;Hibi, M
在斑马鱼和两栖动物的原肠形成过程中，神经外胚层形成了前-后(A-P)模式。我们从斑马鱼中分离到一个新的锌指基因fez-like(Fezl)，该基因与非洲爪哇的fEZ序列具有相似性。从原肠胚晚期(80%胚胎期)到中期，FEZL转录本在神经外胚层的前缘，即预期的背侧前脑中检测到。在这些发育阶段，FEZL也在位于前脊索板上方的腹侧前脑中表达。在表达Wnt抑制因子Dkk1的胚胎中，fezl的表达增强，而在表达Wnt8b的胚胎中，fez1的表达降低。在缺乏前索板的独眼针头突变体和注射抗vin RNA的胚胎中，腹侧前脑的表达被消除。辐射杂交定位表明，fez1基因定位于连锁群II。(C)2000爱思唯尔爱尔兰科学有限公司。保留所有权利。
Anterior-posterior (A-P) patterning in the neuroectoderm is established during gastrulation in zebrafish and amphibians. We isolated a novel zinc-finger gene fez-like (fezl) from zebrafish, which displays sequence similarities to Xenopus Fez. The fezl transcripts were detected in the anterior edge of neuroectoderm, the prospective dorsal forebrain, from the late gastrula (80% epiboly stage) to the mid-segmentation period. fezl was also expressed in the ventral forebrain overlying the prechordal plate at these stages. The expression of fezl was enhanced in embryos expressing the Wnt inhibitor Dkk1 and reduced in embryos expressing Wnt8b. The expression in the ventral forebrain was eliminated in the one-eyed pinhead mutant and the antivin RNA-injected embryos, which lack the prechordal plate. Radiation hybrid mapping revealed that the fezl gene is localized to linkage group ii. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.