Involvement of the cellular prion protein in the migration of brain microvascular e ndothelial cells
Involvement of the cellular prion protein in the migration of brain microvascular e ndothelial cells
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细胞朊病毒蛋白参与脑微血管内皮细胞的迁移
DOI：
10.1016/j.neulet.2011.03.096
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Kata oka Y
中科院分区：
文献类型：
--
作者：
Watanabe T;Yasutaka Y;Nishioku T;Ku sakabe S;Futagami K;Yamauchi A;Kata oka Y
细胞朊病毒蛋白（PrPC）转化为其蛋白酶抗性亚型参与朊病毒病的发病机制。虽然PrPC是一种普遍存在于各种细胞类型中的糖蛋白，但PrPC的生理作用仍不清楚。本研究旨在确定PrPC是否介导脑微血管内皮细胞的迁移。将靶向PrPC的小干扰RNA（siRNA）转染入小鼠脑微血管内皮细胞系（bEND.3细胞）中。siPrP1在三种siRNA中选择，降低bEND.3细胞中PrPC的mRNA和蛋白水平。用划痕试验评价细胞迁移。siPrP1抑制迁移而不显著影响细胞增殖。这项研究提供了第一个证据，证明PrPC可能是脑微血管内皮细胞迁移到脑损伤区域所必需的。PrPC在脑内皮中的这种功能可能是神经血管单位从损伤（如缺血性损伤）中恢复的机制。
The conversion of cellular prion protein (PrPC) to its protease-resistant isoform is involved in the pathogenesis of prion disease. Although PrPCis a ubiquitous glycoprotein that is present in various cell types, the physiological role of PrPCremains obscure. The present study aimed to determine whether PrPCmediates migration of brain microvascular endothelial cells. Small interfering RNAs (siRNAs) targeting PrPCwere transfected into a mouse brain microvascular endothelial cell line (bEND.3 cells). siPrP1, selected among three siRNAs, reduced mRNA and protein levels of PrPCin bEND.3 cells. Cellular migration was evaluated with a scratch-wound assay. siPrP1 suppressed migration without significantly affecting cellular proliferation. This study provides the first evidence that PrPCmay be necessary for brain microvascular endothelial cells to migrate into damaged regions in the brain. This function of PrPCin the brain endothelium may be a mechanism by which the neurovascular unit recovers from an injury such as an ischemic insult.