Sheathless capillary electrophoresis/electrospray mass spectrometry using a carbon-coated tapered fused-silica capillary with a beveled edge
Sheathless capillary electrophoresis/electrospray mass spectrometry using a carbon-coated tapered fused-silica capillary with a beveled edge
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DOI：
10.1021/ac010429o
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发表时间：
2001-11-01
影响因子：
7.4
通讯作者：
Her, GR
中科院分区：
文献类型：
--
作者：
Chang, YZ;Chen, YR;Her, GR
研制了一种用于无鞘毛细管电泳/电喷雾质谱（CE/ESI-MS）的锥形毛细管尖端。75 μ m内径的最佳流速90-mum倾斜锥形毛细管尖端类似于具有25 μ m孔口的常规平锥形尖端。使用黄连碱，小檗碱，和巴马汀氯化物的混合物，鞘CE/ESI-MS的灵敏度的斜面75 μ m锥形尖端毛细管被发现是类似于一个25 μ m的扁平尖端。尽管两种针头都具有相似的CE/ESI-MS灵敏度，但由于外径和内径更大，锥形毛细管针头比传统的25 μ m锥形毛细管更坚固耐用。为了进行电接触，毛细管尖端涂抹油漆标记，然后使用石墨铅笔施加碳涂层。使用这种精细的碳涂层程序，毛细管尖端可以与非质子溶剂一起操作。
A tapered capillary tip containing a beveled edge was developed for use in sheathless capillary electrophoresis/electrospray mass spectrometry (CE/ESI-MS). The optimal flow rate of a 75-mum-i.d., 90-mum-o.d. beveled tapered capillary tip was similar to a conventional flat tapered tip with a 25-mum orifice. Using a mixture of coptisine, berberine, and palmatine chloride, the sheathless CE/ESI-MS sensitivity of a beveled 75 mum tapered tip capillary was found to be similar to a 25 mum flat tip. Although both tips offer similar CE/ESI-MS sensitivity, the beveled tapered capillary tip is more rugged and durable than a conventional 25-mum tapered capillary because of the larger outside diameter and inside diameter. To make electrical contact, the capillary tip was smeared with paint marker followed by the application of a carbon coating using a graphite pencil. Using this refined carbon-coating procedure, the capillary tip can be operated with aprotic solvents.