Introgression of virus-resistance genes into traditional Spanish tomato cultivars (Solanum lycopersicum L.): Effects on yield and quality
Introgression of virus-resistance genes into traditional Spanish tomato cultivars (Solanum lycopersicum L.): Effects on yield and quality
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DOI：
10.1016/j.scienta.2015.11.025
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发表时间：
2016-01
影响因子：
4.3
通讯作者：
F. Rubio;A. Alonso;S. García-Martínez;J. J. Ruiz-J.
中科院分区：
文献类型：
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作者：
F. Rubio;A. Alonso;S. García-Martínez;J. J. Ruiz-J.
本研究的目的是确定和量化ToMV、TSWV和TYLCV抗性基因渗入（分别由tm -2a、sw -5和ty -1基因决定）对新鲜市场番茄产量和品质的影响。以传统番茄品种“De la Pera”和“Muchamiel”为背景，获得了包含3个抗性基因8个纯合组合的2组近等基因系（NILs）。这些NILs在西班牙东南部五个地点的重复试验中种植和评估。在所有进行的分析中，始终具有更大影响的染色体片段是含有ty -1基因的染色体片段，该基因赋予了对TYLCV的抗性。该基因渗入对除可溶性固形物含量外的所有研究参数都产生了负面影响，对总产量和商业产量的影响尤为显著，降低了50%。这种农业产量的减少只有在病毒感染程度高的情况下才能接受。其他引入的片段（含tm -2和sw -5）的影响较小，变化较大，根据试验和研究参数的不同而增加或减少。在纯合子条件下，可以培育出携带ToMV和TSWV抗性基因的高级育种品系，而不会造成农艺性状和品质性状的重大损失。然而，用于抗TYLCV的ty -1基因应仅用于病毒高度感染地区的培育品种。
The objective of this study was to determine and quantify the effects of ToMV, TSWV and TYLCV resistance introgressions (determined byTm-2a,Sw-5andTy-1genes, respectively) on the yield and quality of fresh market tomatoes. Two sets of near isogenic lines (NILs) containing the eight homozygous combinations for the three resistance genes were developed in the backgrounds of the traditional tomato cultivars ‘De la Pera’ and ‘Muchamiel’. These NILs were grown and evaluated in replicated trials in five locations in south-eastern Spain. Across all the analyses performed, the chromosome fragment that consistently had a greater effect was that containing theTy-1gene, which conferred resistance to TYLCV. This gene introgression negatively affected all the studied parameters, except for soluble solids content, and its effect was particularly significant on total and commercial yields, which decreased by 50%. This reduction in agricultural yield would only be acceptable when cultivating under high levels of virus infection. The effects of the other introduced fragments (containingTm-2aandSw-5) were minor and more variable, increasing or decreasing depending on the trial and the studied parameter. Advanced breeding lines carrying ToMV and TSWV resistance genes in homozygous conditions can be developed without important losses in agronomic and quality characteristics. However, theTy-1gene used for TYLCV resistance should only be used in developing cultivars for highly virus-infected areas.