Analysis of the leader and capsid coding regions of persistent and neurovirulent strains of Theiler's virus.
Analysis of the leader and capsid coding regions of persistent and neurovirulent strains of Theiler's virus.
复制标题
泰勒病毒持久性和神经毒力株的前导序列和衣壳编码区的分析。
DOI：
10.1006/viro.1995.0066
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发表时间：
1995
期刊：
影响因子：
3.7
通讯作者：
M. Brahic
中科院分区：
文献类型：
--
作者：
T. Michiels;N. Jarousse;M. Brahic
大多数泰勒病毒株（TMEV）引起小鼠中枢神经系统的持续感染和慢性脱髓鞘疾病，被认为是多发性硬化症的模型。相反，两种菌株会引起急性脑炎，并在几天内杀死老鼠。我们测序的领导人和衣壳编码区的三个持久性（TO4，WW，耶鲁大学）分离株和一个神经毒力（FA）TMEV分离株。我们比较了这些序列和已经发表的其他分离株（DA，BeAn，GDVII和Vilyuisk）。结果表明，毒力和持久性菌株没有演变为两个独立的群体，而是神经毒力菌株从一个亚组的持久性菌株。在不同地理区域和不同时间分离的病毒序列高度同源，这对于RNA病毒来说是一个令人惊讶的发现。这表明在病毒的生命周期中基因组受到了严格的限制。TO4和WW菌株的序列相同，表明后者来自实验室污染。迄今为止测序的所有持久性菌株的基因组在L区含有一个替代的开放阅读框，在DA菌株的情况下，该开放阅读框已被证明编码称为“I”的18 kDa蛋白质。
Most strains of Theiler's virus (TMEV) cause a persistent infection of the central nervous system of the mouse and a chronic demyelinating disease considered a model for multiple sclerosis. Two strains, on the contrary, cause an acute encephalitis and kill mice in a matter of days. We sequenced the leader and capsid coding region of three persistent (TO4, WW, and Yale) isolates and one neurovirulent (FA) isolate of TMEV. We compared these sequences and those already published for other isolates (DA, BeAn, GDVII, and Vilyuisk). The results suggest that virulent and persistent strains did not evolve as two separate groups, but rather that neurovirulent strains arose from a subgroup of persistent strains. The sequences of viruses isolated in different geographic areas and at different times were highly homologous, a surprising finding for an RNA virus. This suggests that severe constraints are imposed on the genome during the viral life cycle. The sequences of the TO4 and WW strains were identical, suggesting that the latter came from a laboratory contamination. The genomes of all the persistent strains sequenced so far contain an alternate open reading frame in the L region, which has been shown, in the case of the DA strain, to code for an 18-kDa protein called "I".