Development and genetic mapping of microsatellite markers from whole genome shotgun sequences in Brassica oleracea
Development and genetic mapping of microsatellite markers from whole genome shotgun sequences in Brassica oleracea
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DOI：
10.1007/s11032-010-9509-y
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发表时间：
2011-12-01
影响因子：
3.1
通讯作者：
Liu, Kede
中科院分区：
文献类型：
--
作者：
Li, Haitao;Chen, Xun;Liu, Kede
油菜全基因组霰弹枪序列（WGSs）的获得为油菜基因组分析和遗传改良的微卫星或SSR标记的开发提供了前所未有的机遇。在本研究中，从甘蓝WGSs中共鉴定出56,465个非冗余SSRs，其中二核苷酸重复序列最多，其次是三、四和五核苷酸重复序列。在此基础上，共开发了1398个重复长度为1 / 1000 y25bp的SSR标记（BoGMS），并用于6个菜籽品种的多态性分析，这是单次研究中最多的C基因组SSR标记。其中752个SSR标记（69.5%）在6个品种间存在多态性。将其中266个在甘蓝型油菜品种2127和ZY821之间表现出明显可分多态性的标记整合到现有的甘蓝型油菜遗传连锁图谱中。这些新标记优先分布在C基因组的连锁群上，显著增加了C基因组的SSR标记数量。这些SSR标记将有助于油菜重要农艺性状的基因定位和标记辅助选择。
The availability of whole genome shotgun sequences (WGSs) in Brassica oleracea provides an unprecedented opportunity for development of microsatellite or simple sequence repeat (SSR) markers for genome analysis and genetic improvement in Brassica species. In this study, a total of 56,465 non-redundant SSRs were identified from the WGSs in B. oleracea, with dinucleotide repeats being the most abundant, followed by tri-, tetra- and pentanucleotide repeats. From these, 1,398 new SSR markers (designated as BoGMS) with repeat length a parts per thousand yen25 bp were developed and used to survey polymorphisms with a panel of six rapeseed varieties, which is the largest number of SSR markers developed for the C genome in a single study. Of these SSR markers, 752 (69.5%) showed polymorphism among the six varieties. Of these, 266 markers that showed clear scorable polymorphisms between B. napus varieties No. 2127 and ZY821 were integrated into an existing B. napus genetic linkage map. These new markers are preferentially distributed on the linkage groups in the C genome, and significantly increased the number of SSR markers in the C genome. These SSR markers will be very useful for gene mapping and marker-assisted selection of important agronomic traits in Brassica species.