Distinguishing between Contact and Administration of Heroin from a Single Fingerprint using High Resolution Mass Spectrometry.
Distinguishing between Contact and Administration of Heroin from a Single Fingerprint using High Resolution Mass Spectrometry.
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使用高分辨率质谱法从单个指纹区分海洛因的接触和施用。
DOI：
10.1093/jat/bkz088
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发表时间：
2020
影响因子：
2.5
通讯作者：
Costa C
中科院分区：
文献类型：
--
作者：
Costa C
指纹已被提出作为一个有前途的新矩阵的药物测试。在以前的工作中，已经表明指纹可以用来区分吸毒者和非吸毒者。在这里，我们看看使用指纹来区分皮肤接触和管理海洛因的可能性。指纹样本收集自（i）10名参加戒毒诊所的患者，（ii）50名非吸毒者和（iii）接触2毫克街头海洛因的参与者，在各种手部清洁程序之前和之后。还从患者体内采集了口腔液。所有样品进行了分析，使用液相色谱-高分辨率质谱法在以前的工作中验证海洛因和6-AM。对吗啡、可待因、6-乙酰可待因和那可丁的HRMS数据进行回顾性分析。海洛因和6-AM被检测到的所有指纹样本从接触海洛因，甚至洗手后。相比之下，吗啡，乙酰可待因和诺斯卡品洗手后成功地删除。在患者样本中，检测到吗啡，诺斯卡品和乙酰可待因（与海洛因和6-AM一起）与患者关于他们最近是否使用海洛因的证词比单独检测海洛因和6-AM更接近。这项研究强调了在捐献指纹样本之前洗手的重要性，以区分最近接触海洛因和使用海洛因。
Fingerprints have been proposed as a promising new matrix for drug testing. In previous work it has been shown that a fingerprint can be used to distinguish between drug users and nonusers. Herein, we look at the possibility of using a fingerprint to distinguish between dermal contact and administration of heroin. Fingerprint samples were collected from (i) 10 patients attending a drug rehabilitation clinic, (ii) 50 nondrug users and (iii) participants who touched 2 mg street heroin, before and after various hand cleaning procedures. Oral fluid was also taken from the patients. All samples were analyzed using a liquid chromatography—high resolution mass spectrometry method validated in previous work for heroin and 6-AM. The HRMS data were analyzed retrospectively for morphine, codeine, 6-acetylcodeine and noscapine. Heroin and 6-AM were detected in all fingerprint samples produced from contact with heroin, even after hand washing. In contrast, morphine, acetylcodeine and noscapine were successfully removed after hand washing. In patient samples, the detection of morphine, noscapine and acetylcodeine (alongside heroin and 6-AM) gave a closer agreement to patient testimony on whether they had recently used heroin than the detection of heroin and 6-AM alone. This research highlights the importance of washing hands prior to donating a fingerprint sample to distinguish recent contact with heroin from heroin use.