EFFECTS OF PARENTERAL PALATINOSE ON GLUCOSE-METABOLISM IN NORMAL AND STREPTOZOTOCIN DIABETIC RATS
EFFECTS OF PARENTERAL PALATINOSE ON GLUCOSE-METABOLISM IN NORMAL AND STREPTOZOTOCIN DIABETIC RATS
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DOI：
10.1055/s-2007-1012317
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发表时间：
1986-06-01
影响因子：
2.2
通讯作者：
YAMASHITA, K
中科院分区：
文献类型：
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作者：
OKUDA, Y;KAWAI, K;YAMASHITA, K
本实验旨在研究大鼠体内帕拉金糖（6-0-α-D-吡喃葡萄糖基-D-果糖）的代谢。在正常和链脲佐菌素 (STZ) 糖尿病大鼠的尾静脉中推注帕拉金糖 (0.5 g/kg) 会导致葡萄糖和胰岛素浓度显着增加。然而，在严重的 STZ 糖尿病大鼠（空腹血糖 > 300 mg/dl）中，没有观察到葡萄糖和胰岛素浓度的显着变化。在肝脏灌注中，通过在灌注液中添加 5.5 mM 帕拉金糖来防止用 20 mM Krebs-Ringer-Tris 缓冲液 pH 7.4 灌注的正常和轻度 STZ 糖尿病大鼠肝脏的葡萄糖输出逐渐减少，并且在帕拉金糖输注期间在流出物中检测到果糖。结果表明，帕拉金糖在正常和糖尿病大鼠组织中代谢为葡萄糖和果糖，这导致血糖浓度升高。另一方面，当将帕拉金糖注入离体灌注大鼠胰腺时，没有观察到胰腺 B 细胞释放胰岛素的直接刺激作用。研究表明，肠胃外施用的帕拉金糖可被组织代谢，有望用作液体和能量供应的来源。
The present experiment was carried out to investigate the metabolism of palatinose (6-0-α-D-glucopyranosyl-D-fructose) in the rat. The bolus injection of palatinose (0.5 g/kg) in the tail vein of normal and Streptozotocin (STZ) diabetic rats caused significant increments in glucose and insulin concentrations. However, in severe STZ diabetic rats (> 300 mg/dl of fasting plasma glucose) no significant change in glucose and insulin concentrations was observed. In liver perfusion, the gradual decrease in glucose output from the normal and mild STZ diabetic rat livers perfused with 20 mM Krebs-Ringer-Tris buffer pH 7.4 was prevented by the addition of 5.5 mM palatinose in the perfusate and fructose was detected in the effluent during the palatinose infusion. The results indicate that palatinose is metabolized to glucose and fructose in both normal and diabetic rat tissues, and this causes the increase in blood glucose concentration. On the other hand, the direct stimulatory effect of insulin release from pancreatic B-cell was not observed when the palatinose was infused into the isolated perfused rat pancreas. The study suggest that palatinose administered parenterally is metabolized by tissues and expected to be used as a source of fluid and energy supply.