Initial quantitative proteomic map of 28 mouse tissues using the SILAC mouse.
Initial quantitative proteomic map of 28 mouse tissues using the SILAC mouse.
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DOI：
10.1074/mcp.m112.024919
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发表时间：
2013-06
期刊：
影响因子：
--
通讯作者：
Mann M
中科院分区：
文献类型：
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作者：
Geiger T;Velic A;Macek B;Lundberg E;Kampf C;Nagaraj N;Uhlen M;Cox J;Mann M
识别哺乳动物组织的组成部分是了解其功能的先决条件。特别是，对哺乳动物组织蛋白质组的全局和定量分析将指出组织特异性机制，并将每种蛋白质的功能置于整个生物体的角度。我们使用高分辨率质谱法对 28 个小鼠组织进行了蛋白质组分析，并使用了通过稳定同位素标记的小鼠组织混合物，并在细胞培养物中用氨基酸作为“掺入”内标，以对这些组织进行准确的蛋白质定量。我们总共鉴定了 7,349 种蛋白质，并对其中 6,974 种蛋白质进行了定量。生物信息数据分析表明，生理相关的组织聚集在一起，高表达的蛋白质代表了特征性的组织功能。组织特化在蛋白质组谱中得到了显着的体现，并且在其一百种最丰富的蛋白质中已经很明显。严格组织特异性蛋白质的比例似乎很小。然而，即使是具有家庭功能的蛋白质，例如核糖体和剪接体中的蛋白质，在组织之间也可能具有显着的表达差异。我们描述了一个计算框架，通过该框架将蛋白质组图谱与组织的生理功能相关联。我们的数据将作为哺乳动物物种蛋白质表达的初始图谱对广大科学界有用。
Identifying the building blocks of mammalian tissues is a precondition for understanding their function. In particular, global and quantitative analysis of the proteome of mammalian tissues would point to tissue-specific mechanisms and place the function of each protein in a whole-organism perspective. We performed proteomic analyses of 28 mouse tissues using high-resolution mass spectrometry and used a mix of mouse tissues labeled via stable isotope labeling with amino acids in cell culture as a “spike-in” internal standard for accurate protein quantification across these tissues. We identified a total of 7,349 proteins and quantified 6,974 of them. Bioinformatic data analysis showed that physiologically related tissues clustered together and that highly expressed proteins represented the characteristic tissue functions. Tissue specialization was reflected prominently in the proteomic profiles and is apparent already in their hundred most abundant proteins. The proportion of strictly tissue-specific proteins appeared to be small. However, even proteins with household functions, such as those in ribosomes and spliceosomes, can have dramatic expression differences among tissues. We describe a computational framework with which to correlate proteome profiles with physiological functions of the tissue. Our data will be useful to the broad scientific community as an initial atlas of protein expression of a mammalian species.