Close arrangement ofCARK3andPMEILaffectsABA-mediated pollen sterility inArabidopsis thaliana
Close arrangement ofCARK3andPMEILaffectsABA-mediated pollen sterility inArabidopsis thaliana
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CARK3和PMEIL的紧密排列影响ABA介导的拟南芥花粉不育
DOI：
10.1111/pce.13871
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发表时间：
2020-09-09
影响因子：
7.3
通讯作者：
Yang, Yi
中科院分区：
文献类型：
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作者：
Wang, Hsi-Hua;Qiu, Yao;Yang, Yi
脱落酸 (ABA) 信号是植物响应胁迫条件的重要信号通路。 ABA 处理可以改变整体基因表达模式并引起显着的表型变化。我们研究了拟南芥开花期间对 ABA 处理的反应。将 CARK3T-DNA 突变体的花浸入 ABA 溶液中，与野生型植物 (Col-0) 相比，花粉育性的降低较少。我们证明，位于 CARK3 下游的基因 PMEIL 直接影响花粉育性。由于CARK3和PMEIL排列紧密，CARK3表达通过转录干扰以ABA依赖性方式抑制PMEIL的转录。我们的研究揭示了 ABA 介导的花粉不育的分子机制，并提供了一个例子，说明基因紧密排列引起的转录干扰如何介导植物繁殖过程中的应激反应。
Abscisic acid (ABA) signaling is a vital plant signaling pathway for plant responses to stress conditions. ABA treatment can alter global gene expression patterns and cause significant phenotypic changes. We investigated the responses to ABA treatment during flowering inArabidopsis thaliana.Dipping the flowers ofCARK3T-DNA mutants in ABA solution, led to less reduction of pollen fertility than in the wild type plants (Col-0). We demonstrated thatPMEIL, a gene located downstream ofCARK3, directly affects pollen fertility. Due to the close arrangement ofCARK3andPMEIL,CARK3expression represses transcription ofPMEILin an ABA-dependent manner through transcriptional interference. Our study uncovers a molecular mechanism underlying ABA-mediated pollen sterility and provides an example of how transcriptional interference caused by close arrangement of genes may mediate stress responses during plant reproduction.