Zonae pellucidae of salt-stored hamster and human eggs: their penetrability by homologous and heterologous spermatozoa.
Zonae pellucidae of salt-stored hamster and human eggs: their penetrability by homologous and heterologous spermatozoa.
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盐储存仓鼠和人卵的透明带：同源和异源精子的渗透性。
DOI：
10.1002/mrd.1120210202
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Lopata,A
中科院分区：
文献类型：
--
作者：
Yoshimatsu,N;Yanagimachi,R;Lopata,A
未受精的仓鼠和人类卵子的透明带在储存于高浓度盐溶液中时仍能被同源精子穿透[Yanagimachi et al.，19791.建议用适当的pH缓冲液中和盐溶液，以长期储存鸡蛋（透明带），因为透明带可能会逐渐膨胀，甚至溶解在酸性或碱性溶液中。至少对仓鼠来说，含有40mMHepes和0.5%葡聚糖的2M硫酸铵溶液（pH7.0）似乎能令人满意地达到目的。在授精前彻底冲洗盐储存的透明带是重要的，但在冲洗介质中加入蛋白酶抑制剂似乎不是必需的。当大量的盐储存鸡蛋必须一次冲洗时，推荐使用透析法。与新鲜（对照）的仓鼠卵带一样，盐储存的仓鼠卵带允许仓鼠精子附着和穿透，但不允许人类精子附着和穿透。另一方面，盐储存的人精子带不仅允许人精子穿透，而且允许仓鼠精子穿透。
Zonae pellucidae of unfertilized hamster and human eggs remain penetrable by homologous spermatozoa when they are stored in highly concentrated salt solutions [Yanagimachi et al., 19791. Neutralization of salt solutions with the appropriate pH buffers is recommended for long-term storage of the eggs (zonae), because zonae may gradually swell or even dissolve in acidic or alkaline solutions. At least for the hamster,’2 M ammonium sulfate solution containing 40 mM Hepes and 0.5% dextran (pH 7.0) seems to serve the purpose satisfactorily. Thorough rinsing of salt-stored zonae prior to insemination is important, but inclusion of protease inhibitors in the rinsing medium does not appear to be essential. A dialysis method is recommended when a large number of salt-stored eggs must be rinsed at one time. Salt-stored hamster zonae, like fresh (control) zonae, permit attachment and penetration of hamster spermatozoa, but not human spermatozoa. Saltstored human zonae, on the other hand, allow penetration by not only human spermatozoa, but also hamster spermatozoa.