SURFACE-PLASMON RESONANCE DETECTION AND MULTISPOT SENSING FOR DIRECT MONITORING OF INTERACTIONS INVOLVING LOW-MOLECULAR-WEIGHT ANALYTES AND FOR DETERMINATION OF LOW AFFINITIES
SURFACE-PLASMON RESONANCE DETECTION AND MULTISPOT SENSING FOR DIRECT MONITORING OF INTERACTIONS INVOLVING LOW-MOLECULAR-WEIGHT ANALYTES AND FOR DETERMINATION OF LOW AFFINITIES
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DOI：
10.1006/abio.1995.1350
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发表时间：
1995-07-01
影响因子：
2.9
通讯作者：
STAHLBERG, R
中科院分区：
文献类型：
--
作者：
KARLSSON, R;STAHLBERG, R
表面等离子体共振检测允许直接观察溶液中分析物与其固定结合伙伴之间的分子相互作用。使用不同数量的固定受体同时监测多个传感表面上的相互作用事件，用于检测低分子量分析物和确定低亲和力进行了研究。采用多点传感和BIAcore 2000仪器，可检测到小至180 Da的分析物，并可确定50 μ M范围内的亲和力。因此，多点传感的使用扩展了直接相互作用分析，包括低分子量分析物和低亲和相互作用。(C) 1995学术出版社，Inc。
Surface plasmon resonance detection allows direct observation of molecular interactions between an analyte in solution and its immobilized binding partner. The use of simultaneous monitoring of interaction events on multiple sensing surfaces, with varying amounts of immobilized receptor, for detection of low-molecular-weight analytes and for determination of low affinities was investigated. Using multispot sensing and BIAcore 2000 instrumentation, analytes as small as 180 Da were detected and affinities in the 50 mu M range could be determined. The use of multispot sensing thus extends direct interaction analysis to include low-molecular-weight analytes and low-affinity interactions. (C) 1995 Academic Press, Inc.