Human ZHX1: cloning, chromosomal location, and interaction with transcription factor NF-Y.
Human ZHX1: cloning, chromosomal location, and interaction with transcription factor NF-Y.
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DOI：
10.1006/bbrc.1999.1087
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发表时间：
1999-08
影响因子：
3.1
通讯作者：
K. Yamada;R. Printz;H. Osawa;D. Granner
中科院分区：
文献类型：
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作者：
K. Yamada;R. Printz;H. Osawa;D. Granner
NF-YA、B和C包含称为核因子Y（NF-Y）或CCAAT结合蛋白（CBF）的异源三聚体转录因子。NF-Y结合许多CCAAT和Y盒（一种倒置的CCAAT盒，ATTGG）元件。这些破坏NF-Y结合的元件的突变导致来自各种组织特异性和诱导型启动子的转录降低。我们采用酵母双杂交系统筛选人肝cDNA文库，以分离与NF-γ相互作用的蛋白质，这些蛋白质可能在组织特异性或可诱导的启动子活性中发挥作用。利用NF-YA亚基的片段作为诱饵，我们分离了编码人类锌指和同源框1（ZHX 1）蛋白的大部分开放阅读框架的cDNA。随后分离出完整的开放阅读框架，并发现其编码一种含有两个锌指和五个同源结构域基序的873个氨基酸的蛋白质。从各种组织中分离的poly（A）（+）RNA的北方印迹分析揭示了约4.5和5个酶的两个主要ZHX 1转录物。这两种转录本普遍表达，尽管5-羟色胺酶转录本在大多数组织中的丰度更高。人类ZHX 1基因位于染色体8q上，在标记CHCL. GATA 50 B06和CHLC之间。GATA 7G07。
NF-YA, B, and C comprise the heterotrimeric transcription factor known as nuclear factor Y (NF-Y) or CCAAT-binding protein (CBF). NF-Y binds many CCAAT and Y box (an inverted CCAAT box, ATTGG) elements. Mutations of these elements that disrupt the binding of NF-Y result in decreased transcription from various tissue-specific and inducible promoters. We employed a yeast two-hybrid system to screen a human liver cDNA library in an effort to isolate proteins that interact with NF-Y and that may play a role in tissue-specific or hormone-inducible promoter activity. Using a fragment of the NF-YA subunit as bait we isolated a cDNA that encodes most of the open reading frame of the human zinc fingers and homeobox 1 (ZHX1) protein. The complete open reading frame was subsequently isolated and found to encode a protein of 873 amino acids that contains two zinc fingers and five homeodomain motifs. Northern blot analysis of poly(A)(+) RNA isolated from various tissues revealed two major ZHX1 transcripts of about 4.5 and 5 kilobases. Both transcripts were expressed ubiquitously, although the 5-kilobase transcript is of greater abundance in most tissues examined. The human ZHX1 gene is located on chromosome 8q, between markers CHCL.GATA50B06 and CHLC. GATA7G07.