Molecular characterization of the dimer formation of Fcα/μ receptor (CD351)
Molecular characterization of the dimer formation of Fcα/μ receptor (CD351)
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Fcα/μ 受体 (CD351) 二聚体形成的分子表征
DOI：
10.1016/j.molimm.2013.04.003
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发表时间：
2013
期刊：
影响因子：
3.6
通讯作者：
Shibuya A
中科院分区：
文献类型：
--
作者：
Takagaki K;Satoh K;Honda S;Shibuya A
Fcα/μR (CD351) 是 IgA 和 IgM 的 Fc 受体，并形成非典型二聚体，可抵抗 2-巯基乙醇或煮沸的还原。我们之前证明 Fcα/μR 的细胞质部分是二聚体形成及其有效细胞表面表达所必需的。然而，这些现象的生化性质尚未确定。通过使用表达 Fcα/μR 细胞质区域缺失突变体的 BW5147 小鼠细胞系，我们发现跨越氨基酸 504-523 的区域是有效细胞表面表达所必需的，而跨越氨基酸 481-490 的区域是二聚体形成所必需的。同时表达Flag标记的Fcα/μR和血凝素标记的Fcα/μR的转染子的免疫印迹分析表明Fcα/μR不形成同二聚体。相反，我们的数据表明 Fcα/μR 与分子量为 60-70kDa 的未知分子形成异二聚体。
Fcα/μR (CD351) is an Fc receptor for both IgA and IgM and forms an atypical dimer that is resistant to reduction by 2-mercaptoethanol or boiling. We previously demonstrated that the cytoplasmic portion of Fcα/μR is required for dimer formation and for its efficient cell-surface expression. However, the biochemical nature of these phenomena has not been determined. By using a BW5147 mouse cell line expressing deletion mutants of the cytoplasmic region of Fcα/μR, we found that the region spanning amino acids 504–523 was required for efficient cell-surface expression, whereas the region spanning amino acids 481–490 was required for dimmer formation. Immunoblotting analyses of transfectants simultaneously expressing Flag-tagged Fcα/μR and hemagglutinin-tagged Fcα/μR suggested that Fcα/μR does not form homodimers. Instead, our data suggest that Fcα/μR forms heterodimers with an as-yet-unknown molecule with a molecular weight of 60–70kDa.