SYNAPTIC RELATIONSHIPS BETWEEN GABA-IMMUNOREACTIVE NEURONS AND AN IDENTIFIED UNIGLOMERULAR PROJECTION NEURON IN THE ANTENNAL LOBE OF PERIPLANETA-AMERICANA - A DOUBLE-LABELING ELECTRON-MICROSCOPIC STUDY
SYNAPTIC RELATIONSHIPS BETWEEN GABA-IMMUNOREACTIVE NEURONS AND AN IDENTIFIED UNIGLOMERULAR PROJECTION NEURON IN THE ANTENNAL LOBE OF PERIPLANETA-AMERICANA - A DOUBLE-LABELING ELECTRON-MICROSCOPIC STUDY
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DOI：
10.1007/bf00271538
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发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
MALUN, D
中科院分区：
文献类型：
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作者：
MALUN, D
用两种特异性标记物的组合标记了美洲大蠊触角叶中的两种类型的中枢神经元。 他们的突触接触的特点和他们的分布在神经元检查。 一个uniglomerular信息素敏感的投射神经元与树突状乔木在男性特定的macroglomerulus（引诱神经元）的特点是生理细胞内记录，然后充满了biocytin，这是转换为一个标记为这个单独的神经元的预嵌入程序。 在植入后的程序本地，多肾小球中间神经元标记的GABA的免疫金标记。 在引诱神经元上发现两种突触接触。 (i)GABA免疫反应谱的输入突触。 有许多这样的，这（连同以前的研究结果）表明，当地interneurons介导的多突触传递从触角受体纤维的投射神经元。 (ii)输出突触到GABA免疫反应谱和未识别的神经元上。 这些接触表明，由特定肾小球中的投射神经元产生的信号被传递回多肾小球中间神经元，因此也被传递到其他肾小球。
Two types of central neurons in the antennal lobe of the American cockroach Periplaneta americana were labeled with a combination of two specific markers. Their synaptic contacts were characterized and their distribution on the neurons examined. A uniglomerular pheromone-sensitive projection neuron with dendritic arbor in the male-specific macroglomerulus (attractant neuron) was characterized physiologically by intracellular recording and then filled with biocytin, which was converted to a marker for this individual neuron by a preembedding procedure. In a postembedding procedure local, multiglomerular interneurons were marked by immunogold labeling of GABA. Two kinds of synaptic contacts were found on the attractant neuron. (i) Input synapses from GABA-immunoreactive profiles. There were many of these, which (together with results of previous studies) suggests that local interneurons mediate polysynaptic transmission from antennal receptor fibers to the projection neuron. (ii) Output synapses onto GABA-immunoreactive profiles and onto non-identified neurons. These contacts indicate that signals generated by the projection neurons in a given glomerulus are passed back to multiglomerular interneurons and hence are also transmitted to other glomeruli.