Bisulfite genomic sequencing-derived methylation profile of the Xist gene throughout early mouse development
Bisulfite genomic sequencing-derived methylation profile of the Xist gene throughout early mouse development
复制标题
DOI：
10.1006/geno.1998.5570
复制
发表时间：
1998-12-15
期刊：
影响因子：
4.4
通讯作者：
Kay, GF
中科院分区：
文献类型：
--
作者：
McDonald, LE;Paterson, CA;Kay, GF
通过甲基化CpG;二核苷酸的差异表观遗传修饰是一种候选机制，其可以鉴定印迹基因的等位基因并导致母本或父本等位基因的单等位基因表达。在配子和早期发育过程中的印记基因的等位基因甲基化状态的测定受到来自这些早期发育阶段的有限数量的基因组DNA的限制。为了避免这个问题，我们使用亚硫酸氢盐基因组测序，以确定在植入前发育过程中的Xist基因内的最小启动子和l-kb区域的等位基因甲基化状态。我们发现，父母Xist等位基因在这些地区没有差异甲基化。我们的研究结果进行了讨论的背景下，以前的相互矛盾的数据获得甲基化敏感的限制性内切酶消化，然后通过PCR扩增甲基化测定。(C)北京：科学出版社.
Differential epigenetic modification by methylation of CpG; dinucleotides is a candidate mechanism that may identify the alleles of imprinted genes and result in monoallelic expression of either the maternal or the paternal allele. Determination of the allelic methylation status of imprinted genes in the gametes and during early development is constrained by the limiting quantities of genomic DNA available from these early developmental stages. To circumvent this problem we have used bisulfite genomic sequencing to determine the allelic methylation status of the minimal promoter and a l-kb region within the Xist gene during preimplantation development. We find that the parental Xist alleles are not differentially methylated in these regions. Our findings are discussed in the context of previous conflicting data obtained using methylation-sensitive restriction enzyme digestion followed by PCR amplification to assay for methylation. (C) 1998 Academic Press.