Metastasis Model to Test the Role of Notch Signaling in Prostate Cancer.
Metastasis Model to Test the Role of Notch Signaling in Prostate Cancer.
复制标题
测试Notch信号在前列腺癌中的作用的转移模型。
DOI：
10.1007/978-1-0716-2201-8_18
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Stoyanova,Tanya
中科院分区：
文献类型：
--
作者：
Liu,Shiqin;Hsu,En-Chi;Shen,Michelle;Aslan,Merve;Stoyanova,Tanya
远处转移是前列腺癌患者死亡的主要原因。Notch信号在前列腺癌侵袭和转移过程中起重要作用。在本章中，我们描述了一种测量前列腺癌转移定植和转移发生率的方案，准确量化转移负担，并测试NOTCH1受体在前列腺癌转移定植和远端归巢中的作用。本研究通过心脏内注射对照人前列腺癌细胞和NOTCH1下调细胞建立转移模型。这些细胞经过工程改造，可以同时表达红色荧光蛋白（RFP）和荧光素酶。在该模型中，利用全身生物发光成像、高分辨率和定量荧光成像来定量评估转移定植和转移负荷。此外，组织病理学分析不同的转移器官进行。该模型是研究NOTCH受体在前列腺癌转移性定植中的功能机制的一个强大而通用的工具。
Distant metastasis is the main cause of death in prostate cancer patients. Notch signaling plays an important role in driving prostate cancer aggressiveness and metastasis. In this chapter, we describe a protocol to measure prostate cancer metastatic colonization, incidences of metastasis, accurately quantify the burden of metastasis, and test the role of NOTCH1 receptor on prostate cancer metastatic colonization and homing to distant sites. The metastasis model presented here is established by intracardiac injection of control human prostate cancer cells and NOTCH1 downregulated cells. The cells are engineered to express both red fluorescent protein (RFP) and luciferase. In this model, whole body bioluminescence imaging, high-resolution, and quantitative fluorescence imaging are utilized for quantitative assessment of metastatic colonization and metastasis burden. Further, histopathology analyses of diverse metastatic organs are performed. This model is a powerful and versatile tool to investigate the mechanisms underlying the function of NOTCH receptors in metastatic colonization in prostate cancer.