Mouse Model for Hepatocellular Carcinoma and Cholangiocarcinoma Originated from Mature Hepatocytes.
Mouse Model for Hepatocellular Carcinoma and Cholangiocarcinoma Originated from Mature Hepatocytes.
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DOI：
10.1007/978-1-4939-8961-4_20
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发表时间：
2018-12
影响因子：
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通讯作者：
Masahiro Yamamoto;B. Xin;Y. Nishikawa
中科院分区：
文献类型：
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作者：
Masahiro Yamamoto;B. Xin;Y. Nishikawa
肝癌由两种主要的组织学亚型组成：肝细胞癌和胆管癌，这两种亚型的预后均较差。因此，在寻找新的治疗靶点时，迫切需要足够的小鼠模型来开发和验证治疗策略。尽管存在肝癌小鼠模型，但每种模型都有缺点。为了克服这些缺点，开发了一种使用水动力尾静脉注射和睡美人转座子的小鼠模型。通过在体内诱导小鼠肝细胞中癌基因的稳定表达，该模型可以很容易地诱发具有取决于用于诱导癌变的癌基因的特定特征的肝癌。在这里，我们详细描述了从小鼠肝细胞诱导肝细胞癌或胆管癌的方法。
Liver cancer consists of two main histological subtypes, hepatocellular carcinoma and cholangiocarcinoma, both of which have poor prognosis. Therefore, in searching for new therapeutic targets, adequate mouse models to develop and validate therapeutic strategies are urgently needed. Although there are mouse models of liver cancer, each model has shortcomings. To overcome these shortcomings, a mouse model using a hydrodynamic tail vein injection and the Sleeping Beauty transposon was developed. By inducing stable expression of oncogenes in mouse hepatocytes in vivo, the model can easily induce liver cancer with specific characteristics that depend on the oncogenes used to induce carcinogenesis. Here, we describe the details of the methods to induce hepatocellular carcinoma or cholangiocarcinoma from mouse hepatocytes.