Review of Hemoglobin-Vesicles as Artificial Oxygen Carriers
Review of Hemoglobin-Vesicles as Artificial Oxygen Carriers
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DOI：
10.1111/j.1525-1594.2008.00698.x
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发表时间：
2009-02-01
影响因子：
2.4
通讯作者：
Tsuchida, Eishun
中科院分区：
文献类型：
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作者：
Sakai, Hiromi;Sou, Keitaro;Tsuchida, Eishun
输血系统极大地促进了人类的健康和福祉。然而，仍然存在一些问题：感染，血型不匹配，免疫反应，保质期短，筛查测试成本。几十年来，血液替代品一直在开发中，以克服这些问题。血浆成分替代品已经建立：血浆膨胀剂，电解质和重组凝血因子。在这里，我们专注于红细胞（RBC）替代品的发展。副作用阻碍了基于无细胞血红蛋白（Hb）的氧载体（HBOCs）的早期发育，并强调了RBC细胞结构的生理重要性。设计良好的人工氧载体，满足所需的标准有望最终实现。血红蛋白的包裹是屏蔽分子血红蛋白毒性的一种想法。然而，必须解决封装的血红蛋白的内在问题：与调节分子组装有关的困难，以及其物理化学和生物化学性质的管理。Hb-vesicles（HbV）是克服这些问题的HBOC的细胞类型。HbV的体内安全性和有效性已被广泛研究。结果说明了HbV作为输血替代品的潜力，并有望用于使用红细胞输血仍然无法实现的其他临床应用。
Blood transfusion systems have greatly benefited human health and welfare. Nevertheless, some problems remain: infection, blood type mismatching, immunological response, short shelf life, and screening test costs. Blood substitutes have been under development for decades to overcome such problems. Plasma component substitutes have already been established: plasma expanders, electrolytes, and recombinant coagulant factors. Herein, we focus on the development of red blood cell (RBC) substitutes. Side effects hindered early development of cell-free hemoglobin (Hb)-based oxygen carriers (HBOCs) and underscored the physiological importance of the cellular structure of RBCs. Well-designed artificial oxygen carriers that meet requisite criteria are expected to be realized eventually. Encapsulation of Hb is one idea to shield the toxicities of molecular Hbs. However, intrinsic issues of encapsulated Hbs must be resolved: difficulties related to regulating the molecular assembly, and management of its physicochemical and biochemical properties. Hb-vesicles (HbV) are a cellular type of HBOC that overcome these issues. The in vivo safety and efficacy of HbV have been studied extensively. The results illustrate the potential of HbV as a transfusion alternative and promise its use for other clinical applications that remain unattainable using RBC transfusion.