Isolation of complementary DNAs encoding a cerebellum-enriched nuclear factor I family that activates transcription from the mouse myelin basic protein promoter.
Isolation of complementary DNAs encoding a cerebellum-enriched nuclear factor I family that activates transcription from the mouse myelin basic protein promoter.
复制标题
分离编码富含小脑的核因子 I 家族的互补 DNA，该家族可激活小鼠髓磷脂碱性蛋白启动子的转录。
DOI：
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发表时间：
1990
影响因子：
4.8
通讯作者：
K. Mikoshiba
中科院分区：
文献类型：
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作者：
Takafumi Inoue;T. Tamura;T. Furuichi;K. Mikoshiba
髓磷脂碱性蛋白（MBP）启动子中的核因子I（NFI）结合序列是脑特异性转录所必需的。在本研究中，我们从小鼠脑cDNA文库中分离了编码NFI家族（NFI-B）的多个cDNA。这些克隆在其5 '、3'或中间部分含有独特的核苷酸序列，推测是可变剪接的结果。NFI-B的整个序列表明NFI-B是NFI-L的小鼠对应物，NFI-L先前从大鼠肝脏中分离。与普遍存在的NFI、CTF/NF-I相比，NFI-B在小脑中高度富集。体外翻译的NFI-B蛋白可与MBP NFI位点结合。此外，我们通过转染实验证明，NFI-B激活从转染的MBP启动子在神经元/神经胶质细胞NG 108 -15细胞中的转录。
A nuclear factor I (NFI) binding sequence in the myelin basic protein (MBP) promoter is required for brain-specific transcription. In the present study, we isolated multiple cDNAs encoding a family of NFI, NFI-B, from mouse brain cDNA libraries. These clones contain unique nucleotide sequences at their 5', 3', or middle portions, presumably as a result of alternative splicing. The entire sequence of NFI-B suggests that NFI-B is a mouse counterpart of NFI-L, which was previously isolated from rat liver. NFI-B is highly enriched in the cerebellum, in contrast with a ubiquitous NFI, CTF/NF-I. NFI-B proteins translated in vitro could bind to the MBP NFI site. Furthermore, we demonstrated by transfection experiments that NFI-B activated transcription from transfected MBP promoter in neuronal/glial NG108-15 cells.