New family of fluorinated polymer chips for droplet and organic solvent microfluidics
New family of fluorinated polymer chips for droplet and organic solvent microfluidics
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DOI：
10.1039/c0lc00356e
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发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Malaquin, Laurent
中科院分区：
文献类型：
--
作者：
Begolo, Stefano;Colas, Guillaume;Malaquin, Laurent
我们推出了一个新的微流控芯片系列，由商用氟化热塑性聚合物 (Dyneon THV) 热压印而成。这种材料具有含氟聚合物的大部分特性（极低的表面能和耐化学品性），但由于其熔点相对较低，更容易加工。最后，作为一种弹性材料，它还可以轻松实现世界与芯片的连接。含氟聚合物薄膜可以通过软光刻制备的 PDMS 模具进行热压印来压印。然后通过原始技术（称为整体粘合粘合）密封芯片，使用两种不同等级的含氟聚合物以获得均匀的机械、化学和表面性能。这种制造工艺非常适合快速原型制作，但它也具有低成本工业生产的潜力，因为它不需要任何固化或蚀刻步骤。我们制备了具有微米分辨率特征的微流体装置，这些装置是光学透明的，并且具有良好的耐压性（高达 50 kPa）。我们演示了水滴在氟化油中的传输以及水滴内 DNA 的荧光检测。没有观察到液滴与通道壁的可测量的相互作用，从而减轻了以前微流控芯片中液滴应用所需的表面处理的需要。这些芯片还可以处理刺激性有机溶剂。例如，我们证明了氟化油中氯仿液滴的形成，扩大了芯片上微化学的潜力。
We present a new family of microfluidic chips hot embossed from a commercial fluorinated thermoplastic polymer (Dyneon THV). This material shares most of the properties of fluoro polymers (very low surface energy and resistance to chemicals), but is easier to process due to its relatively low melting point. Finally, as an elastic material it also allows easy world to chip connections. Fluoropolymer films can be imprinted by hot embossing from PDMS molds prepared by soft lithography. Chips are then sealed by an original technique (termed Monolithic-Adhesive-Bonding), using two different grades of fluoropolymer to obtain uniform mechanical, chemical and surface properties. This fabrication process is well adapted to rapid prototyping, but it also has potential for low cost industrial production, since it does not require any curing or etching step. We prepared microfluidic devices with micrometre resolution features, that are optically transparent, and that provide good resistance to pressure (up to 50 kPa). We demonstrated the transport of water droplets in fluorinated oil, and fluorescence detection of DNA within the droplets. No measurable interaction of the droplets with the channels wall was observed, alleviating the need for surface treatment previously necessary for droplet applications in microfluidic chips. These chips can also handle harsh organic solvents. For instance, we demonstrated the formation of chloroform droplets in fluorinated oil, expanding the potential for on chip microchemistry.