Genome scan linkage analysis identifies a major quantitative trait loci for fatty acid composition in longissimus dorsi muscle in an F(2) intercross between Landrace and Korean native pigs.
Genome scan linkage analysis identifies a major quantitative trait loci for fatty acid composition in longissimus dorsi muscle in an F(2) intercross between Landrace and Korean native pigs.
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DOI：
10.5713/ajas.16.0562
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发表时间：
2017-08
期刊：
影响因子：
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通讯作者：
Cho IC
中科院分区：
文献类型：
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作者：
Park HB;Han SH;Yoo CK;Lee JB;Kim JH;Baek KS;Son JK;Shin SM;Lim HT;Cho IC
本研究对长白猪与韩国地方猪杂交F2代中影响脂肪酸组成的数量性状基因座(QTL)进行定位。对960多个F2代的18个脂肪酸组成性状进行了测定。对所有实验动物进行了分布在猪常染色体上的165个微卫星标记的基因分型。共检测到112个与脂肪酸组成相关的QTL，其中47个达到全基因组显著阈值。特别是，我们在SSC12上发现了一组对FA组成有极显著影响的QTL。猪12号染色体上多不饱和脂肪酸的QTL(加性和显性模型下F值=97.2QTL，标称p值3.6×10−39)可解释表型变异的16.9%。此外，C18：1、C18：2、C20：4和位于相同位置的单不饱和脂肪酸的另外4个QTL解释了10%以上的表型变异。我们发现了一个影响猪FA组成的主效QTL，这可能为定位猪的肉质性状的致病变异提供有用的信息。
This study was conducted to locate quantitative trait loci (QTL) influencing fatty acid (FA) composition in a large F2 intercross between Landrace and Korean native pigs. Eighteen FA composition traits were measured in more than 960 F2 progeny. All experimental animals were genotyped with 165 microsatellite markers located throughout the pig autosomes. We detected 112 QTLs for the FA composition; Forty seven QTLs reached the genome-wide significant threshold. In particular, we identified a cluster of highly significant QTLs for FA composition on SSC12. QTL for polyunsaturated fatty acid on pig chromosome 12 (F-value = 97.2 under additive and dominance model, nominal p-value 3.6×10−39) accounted for 16.9% of phenotypic variance. In addition, four more QTLs for C18:1, C18:2, C20:4, and monounsaturated fatty acids on the similar position explained more than 10% of phenotypic variance. Our findings of a major QTL for FA composition presented here could provide helpful information to locate causative variants to improve meat quality traits in pigs.