Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo.
Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo.
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DOI：
10.1364/ol.35.003195
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发表时间：
2010-10-01
期刊：
影响因子：
3.6
通讯作者：
Wang LV
中科院分区：
文献类型：
--
作者：
Zhang C;Maslov K;Wang LV
光吸收提供了重要的生物学功能信息，但无法被主流光学显微镜技术直接感知，这些技术检测荧光或散射，并且可能需要进行不理想的标记。在此，我们开发了体内亚波长分辨率光声显微镜（SW - PAM），由于非荧光或荧光内源性色素，它能提供极高的光吸收对比度。SW - PAM已接近极限衍射受限的光学分辨率，能够分辨亚细胞器。在未进行标记的情况下，血管系统和早期黑色素瘤分别以12∶1和17∶1的对比度成像。SW - PAM以及放大的宏观成像技术，作为在如此大的长度尺度上测量相同对比度来源的唯一技术，有可能加速从微观研究到临床实践的转化。
Optical absorption provides essential biological functional information but cannot be sensed by mainstream optical microscopy technologies directly, which detect fluorescence or scattering and may require undesirable labeling. Here we developed in vivo subwavelength-resolution photoacoustic microscopy (SW-PAM) that provides exquisitely high optical-absorption contrast due to nonfluorescent, or fluorescent, endogenous pigments. Having approached the ultimate diffraction-limited optical resolution, SW-PAM can resolve subcellular organelles. Vasculature and early-stage melanoma were imaged with 12:1 and 17:1 contrasts, respectively, without labeling. SW-PAM along with the scaled-up macroscopy, as the only technology that measures the same contrast origin over such a wide length scale, can potentially accelerate translation from microscopic research to clinical practice.