Preservation of integrative function in a perfused guinea pig brain.
Preservation of integrative function in a perfused guinea pig brain.
复制标题
保留灌注豚鼠大脑的整合功能。
DOI：
10.1016/0006-8993(90)91001-w
复制
发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Getting,PA
中科院分区：
文献类型：
--
作者：
Richerson,GB;Getting,PA
哺乳动物的脑一直是最难用人工灌注维持的器官之一。正常的生物化学，组织学和脑电生理学已经在体外有限的时间内得到了证明，但要维持复杂的，综合的神经元活动，如脑电图（EEG）或程序化的运动输出，则更加困难。正常的运动输出，除了反射活动，以前没有被证明在灌注脑准备。本文报道了第一个保存正常功能的完整的运动网络，包括完整的传入和传出通路，在灌注的哺乳动物大脑。用含氧载体全氟三丁胺（FC-43）的人工血液原位灌注豚鼠的脑、嘴侧脊髓和周围神经系统。该制备物保持常温，而许多其他灌注脑制备物保持低温以延长存活力。通过向灌注介质中加入HEPES缓冲液，可能通过增加二氧化碳转运来提高存活率。正常脑电图持续时间延长至8h。记录到膈神经自发性呼吸运动输出，其波形和时间模式均正常，平均持续6 h。呼吸运动输出对血二氧化碳分压、温度、血流量、药物浓度和迷走神经传入纤维的电刺激有适当的反应。这种制备代表了在灌注过程中保持神经功能的能力的重大进步，并且应该为研究完整的功能神经网络的细胞电生理学以及神经化学和神经药理学提供优势。
The mammalian brain has been one of the most difficult organs to maintain using artificial perfusion. Normal biochemistry, histology, and electrophysiology of the brain have been demonstrated for limited periods in vitro, but it has been more difficult to maintain complex, integrative neuronal activity such as the electroencephalogram (EEG) or programmed motor output. Normal motor output, other than reflex activity, has not previously been demonstrated in a perfused brain preparation. This paper reports the first preservation of normal function in a complete motor network, including intact afferent and efferent pathways, during perfusion of the mammalian brain. The brain, rostral spinal cord and peripheral nervous system of the guinea pig were perfused in situ using an artificial blood containing the oxygen carrier, perfluorotributylamine (FC-43). This preparation was maintained normothermic, whereas many other perfused brain preparations have been maintained hypothermic to prolong viability. Survival was enhanced by the addition of HEPES buffer to the perfusion medium, probably by increasing carbon dioxide transport. The duration of normal EEG was extended to 8 h. Spontaneous respiratory motor output with normal waveform and temporal pattern was recorded from the phrenic nerve for an average of 6 h. The respiratory motor output responded appropriately to bloodpCO2, temperature, blood flow, drug concentrations, and electrical stimulation of vagal afferent fibers. This preparation represents a significant advance in the ability to preserve neural function during perfusion, and should offer advantages for studying cellular electrophysiology of intact, functioning neural networks, as well as neurochemistry and neuropharmacology.