Growth and fertility of Caenorhabditis elegans (Nematoda) in unpolluted freshwater sediments:: Response to particle size distribution and organic content
Growth and fertility of Caenorhabditis elegans (Nematoda) in unpolluted freshwater sediments:: Response to particle size distribution and organic content
复制标题
DOI：
10.1002/etc.5620181238
复制
发表时间：
1999-12-01
影响因子：
4.1
通讯作者：
Steinberg, CEW
中科院分区：
文献类型：
--
作者：
Höss, S;Haitzer, M;Steinberg, CEW
在沉积物生物测定中，将线虫秀丽隐杆线虫（Maupas）暴露于26种不同的未污染淡水沉积物中，这些未污染淡水沉积物的粒度分布（2.5 - 18%粘土，25.7 - 68.2%粉砂，18.7 - 70.9%砂）和有机物含量（2.5 - 77.1%）各不相同。我们检查了测试终点体长、每只蠕虫的卵和妊娠蠕虫的百分比的变化。秀丽隐杆线虫耐受所有调查的沉积物，至少80%（总平均值96.6%）的蠕虫达到生殖成虫的阶段。体长的变化很小（总平均值为1，235 +/-97.8 μ m），但不同沉积物之间存在显著差异。我们发现体长与粒度分布的相关性很弱，表明线虫在较粗的沉积物中生长得更好。处理间（总平均值12.4 +/-4.8）和处理内（平均值+/-5 - 95%）每只蠕虫的卵数变化相对较大。C.线虫是淡水沉积物生物测定的合适测试生物，使用体长和妊娠蠕虫百分比作为测试终点。
The nematode Caenorhabditis elegans (Maupas) was exposed in a sediment bioassay to 26 different unpolluted freshwater sediments varying in particle size distribution (2.5-18% clay, 25.7-68.2% silt, 18.7-70.9% sand) and organic content (2.5-77.1%). We examined the variation of the test endpoints body length, eggs per worm, and percentage of gravid worms. Caenorhabditis elegans tolerated all investigated sediments, with at least 80% (total mean 96.6%) of the worms reaching the stage of reproductive adults. Variation in body length was small (total mean 1,235 +/- 97.8 mu m), but significant differences among the various sediments were found. We found a weak correlation of body length with particle size distribution, indicating that the nematodes grew better in coarser sediments. The number of eggs per worm showed relatively high variation among treatments (total mean 12.4 +/- 4.8) and also within treatments (mean +/- 5-95%). C. elegans is a suitable test organism for freshwater sediment bioassays, using body length and percentage of gravid worms as test endpoints.