Expression patterns of Slit and Robo family members in adult mouse spinal cord and peripheral nervous system.
Expression patterns of Slit and Robo family members in adult mouse spinal cord and peripheral nervous system.
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Slit和Robo家族成员在成年小鼠脊髓和周围神经系统中的表达模式
DOI：
10.1371/journal.pone.0172736
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Dun XP
中科院分区：
文献类型：
--
作者：
Carr L;Parkinson DB;Dun XP
分泌的糖蛋白Slit 1 -3是经典的轴突引导分子，其通过其充分表征的受体Robo 1 -2作为排斥性线索，以允许精确的轴突寻路和神经元迁移。Slit 1 -3和Robo 1 -2的表达模式在啮齿类动物发育中的神经系统和成年大脑中最具特征，但对其在成年啮齿类动物外周神经系统中的表达模式知之甚少。在这里，我们报告了Slit 1 -3和Robo 1 -2在成年小鼠坐骨神经中的详细表达分析，以及它们在腹侧脊髓（运动神经元）和背根神经节（感觉神经元）内的神经细胞体中的表达。我们的研究结果表明，在成年小鼠周围神经系统中，Slit 1 -3和Robo 1 -2在运动和感觉神经元的细胞体和轴突中表达。Slit 1和Robo 2仅在外周轴突及其胞体中表达，而Slit 2、Slit 3和Robo 1也在背根神经节的卫星细胞、雪旺细胞和外周神经的成纤维细胞中表达。除了这些表达模式，我们还证明了Robo 1在周围神经血管中的表达。我们的工作提供了关于周围神经系统内Slit和Robo家族成员的表达模式的重要新数据，这可能与神经稳态和周围神经对损伤的反应有关。
The secreted glycoproteins, Slit1-3, are classic axon guidance molecules that act as repulsive cues through their well characterised receptors Robo1-2 to allow precise axon pathfinding and neuronal migration. The expression patterns of Slit1-3 and Robo1-2 have been most characterized in the rodent developing nervous system and the adult brain, but little is known about their expression patterns in the adult rodent peripheral nervous system. Here, we report a detailed expression analysis of Slit1-3 and Robo1-2 in the adult mouse sciatic nerve as well as their expression in the nerve cell bodies within the ventral spinal cord (motor neurons) and dorsal root ganglion (sensory neurons). Our results show that, in the adult mouse peripheral nervous system, Slit1-3 and Robo1-2 are expressed in the cell bodies and axons of both motor and sensory neurons. While Slit1 and Robo2 are only expressed in peripheral axons and their cell bodies, Slit2, Slit3 and Robo1 are also expressed in satellite cells of the dorsal root ganglion, Schwann cells and fibroblasts of peripheral nerves. In addition to these expression patterns, we also demonstrate the expression of Robo1 in blood vessels of the peripheral nerves. Our work gives important new data on the expression patterns of Slit and Robo family members within the peripheral nervous system that may relate both to nerve homeostasis and the reaction of the peripheral nerves to injury.