INDEPENDENCE OF STEROIDOGENIC CAPACITY AND LUTEINIZING-HORMONE RECEPTOR INDUCTION IN DEVELOPING GRANULOSA-CELLS
INDEPENDENCE OF STEROIDOGENIC CAPACITY AND LUTEINIZING-HORMONE RECEPTOR INDUCTION IN DEVELOPING GRANULOSA-CELLS
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DOI：
10.1210/endo-102-3-937
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发表时间：
1978-01-01
期刊：
影响因子：
4.8
通讯作者：
ROSS, GT
中科院分区：
文献类型：
--
作者：
HILLIER, SG;ZELEZNIK, AJ;ROSS, GT
研究了FSH（卵泡刺激素）诱导的LH/hCG（促黄体生成素/人绒毛膜促性腺激素）受体的获得与雌激素致敏的垂体切除大鼠卵巢颗粒细胞类固醇生成能力之间的关系。从未用FSH处理的动物（对照组）的未成熟腔前卵泡中收获的颗粒细胞显示出可忽略的特异性人[125 I]碘-hCG结合，并且在体外培养48小时期间仅产生极少量的孕酮。向培养基中加入高度纯化的hFSH或前列腺素E2（PGE 2）引起孕酮产生的显著增加，但不伴随[125 I]碘-hCG结合的可测量增加。用o[绵羊]FSH在体内处理24小时导致许多卵泡开始形成窦，并伴随着新鲜分离的颗粒细胞的hCG结合增加8-10倍。培养过程中基础hFSH和PGE 2刺激的孕酮产生也大于对照组。从动物体内接受促卵泡激素仅12小时的细胞显示，无论是培养前或培养后的hCG结合没有增加，但基础和刺激孕酮的生产在体外显着大于对照组，表明类固醇激素的启动是先行LH/hCG受体诱导。在hCG存在下孵育时，只有那些用oFSH体内处理24 h后获得的细胞产生升高量的孕酮，从而表明观察到的[125 I]碘-hCG结合增加反映了功能活性LH/hCG受体的诱导。药理刺激类固醇激素的细胞悬浮液与N，O“-二丁酰c[环]AMP导致一致的高水平的孕酮生产，无论以前的治疗与FSH在体内。这种体外类固醇生成能力的统一表达发生在完全没有可测量的LH/hCG受体增加的情况下，表明这2个基本的发育过程是独立的现象，可能在体内受到单独的调节。
The relationship between FSH[follicle stimulating hormone]-induced acquisition of LH/hCG [luteinizing hormone/human chorionic gonadotropin] receptors and the steroidogenic capacity of granulosa cells from estrogen-primed hypophysectomized rat ovaries was examined. Granulosa cells harvested from the immature preantral follicles of animals not treated with FSH (controls) displayed negligible specific human [125I]iodo-hCG binding and produced only minimal amounts of progesterone during 48 h of culture in vitro. Addition of highly purified hFSH or prostaglandin-E2 (PGE2) to the culture medium elicited substantial increases in progesterone production which were not accompanied by measurable increases in [125I]iodo-hCG binding. Treatment with o[ovine]FSH in vivo for 24 h led to the initiation of antrum formation in many follicles and was accompanied by an 8-10-fold increase in hCG binding by freshly isolated granulosa cells. Basal hFSH- and PGE2-stimulated progesterone production during culture was also greater than controls. Cells from animals receiving oFSH in vivo for only 12 h showed no increase in hCG binding either before or after culture, yet basal and stimulated progesterone production in vitro was significantly greater than controls, indicating that the initiation of steroidogenesis was antecedent to LH/hCG receptor induction. Only those cells obtained after the 24 h in vivo treatment with oFSH produced elevated amounts of progesterone when incubated in the presence of hCG, thereby showing that the observed increases in [125I]iodo-hCG binding reflected the induction of functionally active LH/hCG receptors. Pharmacological stimulation of steroidogenesis by cell suspensions with N,O''-dibutyryl c[cyclic]AMP resulted in consistently high levels of progesterone production irrespective of previous treatment with FSH in vivo. This uniform expression of in vitro steroidogenic capacity occurred in the complete absence of measurable increases in LH/hCG receptors, suggesting that these 2 fundamental developmental processes are independent phenomena which may be under separate regulation in vivo.