Microwave-assisted aqueous enzymatic extraction of oil from Isatis indigotica seeds and its evaluation of physicochemical properties, fatty acid compositions and antioxidant activities
Microwave-assisted aqueous enzymatic extraction of oil from Isatis indigotica seeds and its evaluation of physicochemical properties, fatty acid compositions and antioxidant activities
复制标题
DOI：
10.1016/j.indcrop.2012.12.050
复制
发表时间：
2013-02
影响因子：
5.9
通讯作者：
Qing-Yan Gai;Jiao Jiao-Jiao;Pan-Song Mu;Wei Wang;Meng Luo;Chunying Li;Y. Zu;Fu-Yao Wei;Yu-jie Fu
中科院分区：
文献类型：
--
作者：
Qing-Yan Gai;Jiao Jiao-Jiao;Pan-Song Mu;Wei Wang;Meng Luo;Chunying Li;Y. Zu;Fu-Yao Wei;Yu-jie Fu
研究了一种新颖、绿色的微波辅助水相酶萃取板蓝籽油的方法。复合酶(纤维素酶/蛋白酶/果胶酶=1/1/1，w/w/w)对油脂的释放效果最好。在酶浓度为1.82%(w/w)、温度为43℃、时间为83min、辐射功率为375W的最佳工艺条件下，最高采油率为59.27%。与传统的索氏提取油(SEO)相比，MAAEE油(MAAEEO)具有相似的物理化学性质，但具有更好的氧化安定性。DPPH自由基清除试验和β-胡萝卜素/亚油酸漂白试验表明，MAAEEO具有较高的抗氧化活性，IC50值分别为9.58和15.03 mg/mL。扫描电子显微镜显示了MAAEE对样品细胞壁和细胞膜结构的严重破坏。
A novel and green microwave-assisted aqueous enzymatic extraction (MAAEE) of oil from Isatis indigotica seeds was investigated in the present study. Enzyme cocktail (cellulase/proteinase/pectinase=1/1/1, w/w/w) was found to be the most effective in releasing the oil. Under the optimal conditions as enzyme concentration 1.82% (w/w), temperature 43°C, time 83min and irradiation power 375W, the highest oil recovery of 59.27% was obtained using the emerging technique. As compared to conventional Soxhlet extraction-derived oil (SEO), MAAEE-derived oil (MAAEEO) revealed the similar physicochemical properties but exhibited better oxidation stability. In addition, MAAEEO had higher contents of valuable polyunsaturated fatty acids, tocopherols and phenolics as against SEO, and it exhibited considerable antioxidant activities with IC50values 9.58 and 15.03mg/mL, according to DPPH radical scavenging assay and β-carotene/linoleic acid bleaching test, respectively. Scanning electron micrographs illustrated the severely structural disruptions of sample cell walls and membranes by MAAEE.