C-ERBB-2 AMPLIFICATION AND OVEREXPRESSION IN BREAST-CANCER - EVALUATION AND COMPARISON OF SOUTHERN BLOT, SLOT BLOT, ELISA AND IMMUNOHISTOCHEMISTRY
C-ERBB-2 AMPLIFICATION AND OVEREXPRESSION IN BREAST-CANCER - EVALUATION AND COMPARISON OF SOUTHERN BLOT, SLOT BLOT, ELISA AND IMMUNOHISTOCHEMISTRY
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DOI：
10.3109/00313029309084784
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发表时间：
1993-04-01
期刊：
影响因子：
4.5
通讯作者：
STERRETT, GF
中科院分区：
文献类型：
--
作者：
DAWKINS, HJS;ROBBINS, PD;STERRETT, GF
癌基因 c-erbB-2 已被证明在 17-30% 的乳腺癌中扩增，癌基因产物 p185（一种跨膜生长因子受体糖蛋白）的过度表达水平相似。 c-erbB-2 扩增现在通常被认为是乳腺癌患者的重要预后指标。对一系列 74 个连续乳腺癌进行了 c-erbB-2 扩增和 p185 过表达分析。采用Southern印迹法和狭缝印迹法分析癌基因扩增情况，采用免疫过氧化物酶（IPOX）染色和酶联免疫吸附试验（ELISA）分析p185过表达。常规Southern印迹法和狭缝印迹法检测c-erbB-2癌基因扩增结果完全一致，74例患者中有14例检测到扩增的c-erbB-2癌基因。 （18.9%）。通过IPOX和ELISA检测，发现c-erbB-2癌蛋白分别在21%和19%的患者中过度表达。对所有四种方法获得的结果进行比较，并从易用性、费用和引入常规诊断实验室的难易程度对技术进行进一步比较。免疫细胞化学与狭缝印迹程序相结合被认为是在常规病理学实验室中评估过表达和扩增的最具成本效益的方法。
The oncogene c-erbB-2 has been shown to be amplified in 17-30% of breast cancers, with similar levels of overexpression of the oncogene product p185, a transmembrane growth factor receptor glycoprotein. Amplification of c-erbB-2 is now generally considered to be a significant prognostic indicator in patients with breast cancer.A series of 74 consecutive breast carcinomas were analysed for c-erbB-2 amplification and p185 overexpression. The procedures of Southern blotting and slot blot were used for the analysis of oncogene amplification, while immunoperoxidase (IPOX) staining and enzyme-linked immunosorbent assay (ELISA) were used for the analysis of p185 overexpression.Detection of c-erbB-2 oncogene amplification by both the conventional Southern blotting technique and by the slot blot technique showed complete accord, with the amplified c-erbB-2 oncogene being detected in 14 of the 74 patients (18.9%). The c-erbB-2 oncoprotein, as measured by IPOX and ELISA, was found to be overexpressed in 21% and 19% of patients, respectively.Comparison was made between the results attained by all four methods, and further comparison of the techniques was made from the point of view of ease of use, expense and ease of introduction into routine diagnostic laboratories. Immunocytochemistry in combination with slot blotting procedures were considered to be the most cost effective methods for evaluation of overexpression and amplification in routine pathology laboratories.