Nanocrystalline hydroxyapatite/titania coatings on titanium improves osteoblast adhesion.
Nanocrystalline hydroxyapatite/titania coatings on titanium improves osteoblast adhesion.
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DOI：
10.1002/jbm.a.31469
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
Michiko Sato;A. Aslani;Marisa A. Sambito;N. Kalkhoran;E. Slamovich;T. Webster
中科院分区：
文献类型：
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作者：
Michiko Sato;A. Aslani;Marisa A. Sambito;N. Kalkhoran;E. Slamovich;T. Webster
块状羟基磷灰石（HA）和二氧化钛已被用于改善骨科植入物的骨整合。由于这个原因，HA和二氧化钛的复合材料作为新型涂层材料在骨科领域受到越来越多的关注。该体外研究的目的是产生纳米相（即，晶粒尺寸小于100 nm的材料）钛上的HA/二氧化钛涂层。骨形成细胞（成骨细胞）在复合涂层上的粘附也进行了评估，并与单相纳米二氧化钛和纳米HA钛涂层进行了比较。采用湿化学法和水热法在200 ℃下合成了纳米HA粉体。将商业上获得的纳米晶二氧化钛粉末与纳米晶HA粉末以各种重量比混合。然后利用称为IonTite的室温涂层工艺将混合粉末沉积在钛上。本研究的结果表明，这样的涂层保持了原来的HA和二氧化钛粉末的化学和微晶尺寸。此外，成骨细胞粘附在单相纳米二氧化钛涂层上是良好的扩散，而它们在复合涂层和单相HA涂层上变得更圆，并延伸出明显的丝状伪足。有趣的是，与单相纳米二氧化钛涂层、目前使用的等离子喷涂HA涂层和未涂覆的钛相比，在重量比为2/1和1/2的纳米二氧化钛/HA复合涂层上粘附的成骨细胞的数量显著更大。这些研究结果表明，纳米二氧化钛/HA涂层钛应进一步研究，以改善骨科应用。
Bulk hydroxyapatite (HA) and titania have been used to improve the osseointegration of orthopedic implants. For this reason, composites of HA and titania have been receiving increased attention in orthopedics as novel coating materials. The objective of this in vitro study was to produce nanophase (i.e., materials with grain size less than 100 nm) HA/titania coatings on titanium. The adhesion of bone forming cells (osteoblasts) on the composite coatings were also assessed and compared with single-phase nanotitania and nano-HA titanium coatings. Nanocrystalline HA powders were synthesized through wet chemistry and hydrothermal treatments at 200 degrees C. Nanocrystalline titania powders obtained commercially were mixed with the nanocrystalline HA powders at various weight ratios. The mixed powders were then deposited on titanium utilizing a room-temperature coating process called IonTite. The results of the present study showed that such coatings maintained the chemistry and crystallite size of the original HA and titania powders. Moreover, osteoblasts adherent on single-phase nanotitania coatings were well-spread whereas they became more round and extended distinct filopodia on the composite and single-phase HA coatings. Interestingly, the number of osteoblasts adherent on the nanotitania/HA composite coatings at weight ratios of 2/1 and 1/2 were significantly greater compared with single-phase nanotitania coatings, currently-used plasma-sprayed HA coatings, and uncoated titanium. These findings suggest that nanotitania/HA coatings on titanium should be further studied for improved orthopedic applications.