Expression of NTPDase1 and NTPDase2 in murine kidney: relevance to regulation of P2 receptor signaling.
Expression of NTPDase1 and NTPDase2 in murine kidney: relevance to regulation of P2 receptor signaling.
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小鼠肾脏中 NTPDase1 和 NTPDase2 的表达：与 P2 受体信号传导调节的相关性。
DOI：
10.1152/ajprenal.00108.2004
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Robson,SimonC
中科院分区：
文献类型：
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作者：
Kishore,BellamkondaK;Isaac,Jorge;Fausther,Michel;Tripp,SherylR;Shi,Huihui;Gill,PritmohinderS;Braun,Norbert;Zimmermann,Herbert;Sévigny,Jean;Robson,SimonC
细胞外核苷酸对肾功能的调节包括肾小球血流动力学、微血管功能、肾小管肾小球反馈、肾小管转运、细胞生长或凋亡以及髓集合管中水和溶质的转运的改变。几乎所有的细胞都可以释放ATP或其他核苷酸，然后在细胞外环境中迅速水解。然而，很少有信息是可利用的细胞外酶的细胞表达，水解细胞外核苷酸在肾脏内。核苷三磷酸二磷酸水解酶（NTPDases）是质膜结合的外核苷酸酶。NTPD酶1与B淋巴细胞活化标志物CD 39具有同一性，并在血管系统内将细胞外ATP和ADP水解为AMP，而NTPD酶2/CD 39 L（ike）1优先在血管外将ATP转化为ADP。利用免疫组织化学和原位杂交方法，我们定位的蛋白和mRNA的NTPDase 1和2在小鼠肾组织。在肾皮质中，NTPD酶1由小叶间动脉、传入肾小球小动脉和管周毛细血管中的血管平滑肌细胞和内皮细胞表达。在髓质内，NTPD酶1表达于亨利氏袢、贝里尼管和骨盆壁的上升细肢中。相比之下，NTPD酶2在鲍曼氏囊、肾小球小动脉、血管外膜和骨盆壁中表达。因此，NTPD酶的分布模式与已知的P2受体在肾脏内的分布相似。NTPD酶可以调节ATP和降解产物在血管系统和其他部位内的调节作用，从而潜在地影响肾脏中的生理以及多种病理事件。
The regulation of renal function by extracellular nucleotides encompasses alterations in glomerular hemodynamics, microvascular function, tubuloglomerular feedback, tubular transport, cell growth or apoptosis, and transport of water and solutes in the medullary collecting duct. Nearly all cells can release ATP or other nucleotides that are then rapidly hydrolyzed in the extracellular milieu. However, little information is available on the cellular expression of ectoenzymes that hydrolyze extracellular nucleotides within the kidney. Nucleoside triphosphate diphosphohydrolases (NTPDases) are plasma membrane-bound ectonucleotidases. NTPDase1 has identity with CD39, a B lymphocyte activation marker, and hydrolyzes extracellular ATP and ADP to AMP within the vasculature, whereas NTPDase2/CD39L(ike)1 preferentially converts ATP to ADP outside of blood vessels. Using immunohistochemical and in situ hybridization approaches, we localized the protein and mRNA of NTPDase1 and 2 in murine renal tissues. In the renal cortex, NTPDase1 is expressed by vascular smooth muscle cells and endothelium in interlobular arteries, afferent glomerular arterioles, and peritubular capillaries. In the inner medulla, NTPDase1 is expressed in ascending thin limbs of Henle's loop, ducts of Bellini, and in the pelvic wall. In contrast, NTPDase2 is expressed in Bowman's capsule, glomerular arterioles, adventitia of blood vessels, and pelvic wall. Thus the distribution patterns of NTPDases have parallels to the known distribution of P2 receptors within the kidney. NTPDases may modulate regulatory effects of ATP and degradation products within the vasculature and other sites and thereby potentially influence physiological as well as multiple pathological events in the kidney.