Interference-free determination of and in plant samples using excitation-emission matrix fluorescence based on oxidationderivatization coupled with second-order calibration methods.
Interference-free determination of and in plant samples using excitation-emission matrix fluorescence based on oxidationderivatization coupled with second-order calibration methods.
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DOI：
10.1039/b9ay00048h
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发表时间：
2009-11
期刊：
影响因子：
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通讯作者：
Yuan-Na Li;Hai-Long Wu;Jinfang Nie;Shu-Fang Li;Yong-Jie Yu;Shu-Rong Zhang;R. Yu
中科院分区：
文献类型：
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作者：
Yuan-Na Li;Hai-Long Wu;Jinfang Nie;Shu-Fang Li;Yong-Jie Yu;Shu-Rong Zhang;R. Yu
建立了一种同时测定银杏叶片和花蕾提取液中脱落酸（阿坝）和赤霉素（GA）含量的二阶校正激发发射荧光法。该方法是基于交替归一化加权误差（ANWE）和并行因子分析（PARAFAC）算法，这使得它有可能，阿坝和GA的浓度可以达到植物提取物中，即使在存在未知的干扰，从潜在的干扰基质污染物在简单的预处理过程中引入。虽然分析物的激发和发射谱彼此之间以及提取物中的背景存在严重重叠，但获得了令人满意的回收率。叶片样品中GA和阿坝的检测限分别为9.6和6.9 ng/mL，在不同时期叶片中GA和阿坝的浓度范围（从几百到几ng/g）。此外，为了研究所开发的方法的性能，一些统计参数和优值的ANWE和PARAFAC进行了评估。该方法为植物提取物中植物激素的定量分析提供了一条新的途径，具有简单的前处理过程，并有可能在植物生长过程中得到更实际的应用。
A sensitive excitation-emission fluorescence method with a second-order calibration strategy is proposed to simultaneously determine abscisic acid (ABA) and gibberellin (GA) contents in extracts of leaves and buds of ginkgo. The methodology is based on the alternating normalization-weighed error (ANWE) and the parallel factor analysis (PARAFAC) algorithms, which make it possible that the ABA and GA concentration can be attained in extract of plants even in the presence of unknown interference from potential interfering matrix contaminants introduced during the simple pretreatment procedure. Satisfactory recoveries were obtained although the excitation and emission profiles of the analytes were heavily overlapped with each other and the background in the extracts. The limits of detection obtained for GA and ABA in leaf samples were 9.6 and 6.9 ng mL-1, respectively, which were in the concentration range (from hundreds to several ng g-1) for GA and ABA in leaves in different periods. Furthermore, in order to investigate the performance of the developed method, some statistical parameters and figures of merit of ANWE and PARAFAC are evaluated. The method proposed lights a new avenue to determine quantitatively phytohormones in extracts of plants with a simple pretreatment procedure, and may hold potential to be extended as a promising alternative for more practical applications in plant growth processes.