Interaction of viral pathogen with porin channels on the outer membrane of insect bacterial symbionts mediates their joint transovarial transmission
Interaction of viral pathogen with porin channels on the outer membrane of insect bacterial symbionts mediates their joint transovarial transmission
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DOI：
10.1098/rstb.2018.0320
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发表时间：
2019-01
期刊：
影响因子：
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通讯作者：
Wei Wu;Lingzhi Huang;Qianzhuo Mao;Jing Wei;Jiajia Li;Yu Zhao;Qian Zhang;Dongsheng Jia;T. Wei
中科院分区：
文献类型：
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作者：
Wei Wu;Lingzhi Huang;Qianzhuo Mao;Jing Wei;Jiajia Li;Yu Zhao;Qian Zhang;Dongsheng Jia;T. Wei
许多能够以持久和跨生殖方式传播植物病毒的半足类昆虫通常与一个共同的专性细菌共生体Sulcia及其β-变形菌伙伴有关。水稻矮病毒（Rice dwarf virus， RDV）是一种植物呼肠孤病毒，通过病毒次要外衣壳蛋白P2与细菌外膜蛋白的直接相互作用，结合到Sulcia的包膜上，使病毒能够利用水稻叶蝉载体中Sulcia的古卵母细胞进入途径。在这里，我们发现RDV可以与Sulcia及其β-变形菌伙伴Nasuia搭便车，以确保它们同时经卵巢传播。有趣的是，RDV可以穿过Nasuia的外膜并驻留在质周空间，这是由病毒主要外衣壳蛋白P8与细菌外膜上的孔蛋白通道的特异性相互作用介导的。蝇孔特异性抗体有效地干扰RDV与蝇包膜的结合，从而有效地阻止病毒向昆虫后代传播。因此，RDV进化出了不同的策略来利用稻叶蝉中两种专性细菌共生体使用的古老卵母细胞进入路径。因此，我们的研究结果表明，RDV与Sulcia和Nasuia在其联合经卵巢传播过程中形成了复杂的合作相互作用。这篇文章是主题问题“生物信号揭示智能害虫管理”的一部分。
Many hemipteran insects that can transmit plant viruses in a persistent and transovarial manner are generally associated with a common obligate bacterial symbiont Sulcia and its β-proteobacterial partner. Rice dwarf virus (RDV), a plant reovirus, can bind to the envelope of Sulcia through direct interaction of the viral minor outer capsid protein P2 with the bacterial outer membrane protein, allowing the virus to exploit the ancient oocyte entry path of Sulcia in rice leafhopper vectors. Here, we show that RDV can hitchhike with both Sulcia and its β-proteobacterial partner Nasuia to ensure their simultaneous transovarial transmission. Interestingly, RDV can move through the outer envelope of Nasuia and reside in the periplasmic space, which is mediated by the specific interaction of the viral major outer capsid protein P8 and the porin channel on the bacterial outer envelope. Nasuia porin-specific antibody efficiently interferes with the binding between RDV and the Nasuia envelope, thus strongly preventing viral transmission to insect offspring. Thus, RDV has evolved different strategies to exploit the ancient oocyte entry paths used by two obligate bacterial symbionts in rice leafhoppers. Our results thus reveal that RDV has formed complex, cooperative interactions with both Sulcia and Nasuia during their joint transovarial transmission. This article is part of the theme issue ‘Biotic signalling sheds light on smart pest management’.