Evaluation of serum estrogen-DNA adducts as potential biomarkers for breast cancer risk.
Evaluation of serum estrogen-DNA adducts as potential biomarkers for breast cancer risk.
复制标题
评估血清雌激素-DNA加合物作为乳腺癌风险的潜在生物标志物。
DOI：
10.1016/j.jsbmb.2012.02.002
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发表时间：
2012-10
影响因子：
4.1
通讯作者：
Rogan, Eleanor G.
中科院分区：
文献类型：
--
作者：
Pruthi, Sandhya;Yang, Li;Sandhu, Nicole P.;Ingle, James N.;Beseler, Cheryl L.;Suman, Vera J.;Cavalieri, Ercole L.;Rogan, Eleanor G.
这项研究旨在确定早发性乳腺癌患者和中高风险乳腺癌患者血清中雌激素-DNA加合物与其各自的代谢物和结合物的比率是否存在差异。用UPLC-MS/MS分析63例乳腺癌中危或80例高危乳腺癌患者(Gail模型)和79例初诊早期乳腺癌患者的血清标本，比较三组间的加合物比率，并用受试者工作特征曲线(AUC)下的面积确定诊断界点。中危组的中位加合物比显著低于高危组和乳腺癌组(P<0.0001.0 1)，并能很好地区分乳腺癌的中危组和高危组(AUC=0.84，95%CI 0.77~0.90)。当加成比为77时，灵敏度和特异度达到最大。对于相同年龄和体重指数组的女性，比值至少为77的女性患乳腺癌的高危几率是比值小于77的女性的8.03(95%可信区间3.46-18.7)倍。与低加合物比率的人相比，高加合物比率的人患乳腺癌的高风险的可能性显著增加。这些发现表明，雌激素-DNA加合物作为乳腺癌风险的潜在生物标记物值得进一步研究。
This study was conducted to determine whether the ratio of estrogen-DNA adducts to their respective metabolites and conjugates in serum differed between women with early-onset breast cancer and those with average or high risk of developing breast cancer. Serum samples from women at average risk (n = 63) or high risk (n = 80) for breast cancer (using Gail model) and women newly diagnosed with early breast cancer (n = 79) were analyzed using UPLC-MS/MS. Adduct ratios were statistically compared among the three groups, and the Area Under the Receiver Operating Characteristic Curve (AUC) was used to identify a diagnostic cut-off point. The median adduct ratio in the average-risk group was significantly lower than that of both the high-risk group and the breast cancer group (p values <0.0001), and provided good discrimination between those at average versus high risk of breast cancer (AUC = 0.84, 95% CI 0.77–0.90). Sensitivity and specificity were maximized at an adduct ratio of 77. For women in the same age and BMI group, the odds of being at high risk for breast cancer was 8.03 (95% CI 3.46–18.7) times higher for those with a ratio of at least 77 compared to those with a ratio less than 77. The likelihood of being at high risk for breast cancer was significantly increased for those with a high adduct ratio relative to those with a low adduct ratio. These findings suggest that estrogen-DNA adducts deserve further study as potential biomarkers for risk of developing breast cancer.
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影响因子：
3.8
作者：
Wen, Wanqing;Ren, Zefang;Zheng, Wei
通讯作者：
Zheng, Wei
DOI：
10.1016/j.jsbmb.2006.12.102
发表时间：
2007-06-01
影响因子：
4.1
作者：
Lu, Fang;Zahid, Muhammad;Rogan, Eleanor G.
通讯作者：
Rogan, Eleanor G.
影响因子：
7.4
作者：
Gaikwad, Nilesh W.;Rogan, Eleanor G.;Cavalieri, Ercole L.
通讯作者：
Cavalieri, Ercole L.
影响因子：
4.1
作者：
Cavalieri, EL;Kumar, S;Rogan, EG
通讯作者：
Rogan, EG
影响因子：
7.4
作者：
Zahid, Muhammad;Saeed, Muhammad;Cavalieri, Ercole
通讯作者：
Cavalieri, Ercole