Effects of glutathione reductase inhibition on cellular thiol redox state and related systems.
Effects of glutathione reductase inhibition on cellular thiol redox state and related systems.
复制标题
DOI：
10.1016/j.abb.2009.03.001
复制
发表时间：
2009-05-01
影响因子：
3.9
通讯作者：
Guan X
中科院分区：
文献类型：
--
作者：
Zhao Y;Seefeldt T;Chen W;Wang X;Matthees D;Hu Y;Guan X
虽然谷胱甘肽还原酶（GR）的抑制已被证明会导致还原型谷胱甘肽（GSH）的减少和谷胱甘肽二硫化物（GSSG）的增加，但尚未注意到GR抑制对巯基氧化还原状态和相关系统的影响的系统研究。以猴肾细胞为模型，以2-乙酰氨基-3-[4-（2-乙酰氨基-2-羧基乙硫基-羰基氨基）苯硫氨甲酰基硫基]丙酸（2-AAPA）为GR抑制剂，研究GR抑制对细胞巯基氧化还原状态及相关系统的影响。我们的研究表明，除了GSH减少和GSSG增加外，2-AAPA还增加了NADH/NAD+和NADPH/NADP+的比值。观察到显著的蛋白谷胱甘肽化。然而，这种抑制并不影响活性氧的形成或抗氧化防御酶系统[GR、谷胱甘肽过氧化物酶、过氧化氢酶和超氧化物歧化酶]和参与GSH生物合成的酶[γ-谷氨酰半胱氨酸合成酶和谷胱甘肽合成酶]的表达。
Although inhibition of glutathione reductase (GR) has been demonstrated to cause a decrease in reduced glutathione (GSH) and increase in glutathione disulfide (GSSG), a systematic study of the effects of GR inhibition on thiol redox state and related systems has not been noted. By employing a monkey kidney cell line as the cell model and 2-acetylamino-3-[4-(2-acetylamino-2-carboxy-ethylsulfanylthio-carbonylamino)phenylthiocarbamoylsulfanyl]propionic acid (2-AAPA) as a GR inhibitor, an investigation of the effects of GR inhibition on cellular thiol redox state and related systems was conducted. Our study demonstrated that, in addition to a decrease in GSH and increase in GSSG, 2-AAPA increased the ratios of NADH/NAD+ and NADPH/NADP+. Significant protein glutathionylation was observed. However, the inhibition did not affect the formation of reactive oxygen species or expression of antioxidant defense enzyme systems [GR, glutathione peroxidase, catalase, and superoxide dismutase)] and enzymes involved in GSH biosynthesis [γ-glutamylcysteine synthetase and glutathione synthetase].
登录
查看更多内容
DOI：
10.1016/j.jpba.2008.08.033
发表时间：
2008-12-15
影响因子：
3.4
作者：
Chen, Wei;Zhao, Yong;Seefeldt, Teresa;Guan, Xiangming
通讯作者：
Guan, Xiangming
影响因子：
4.8
作者：
Chrestensen, CA;Starke, DW;Mieyal, JJ
通讯作者：
Mieyal, JJ
影响因子：
4.8
作者：
Aon, Miguel A.;Cortassa, Sonia;O'Rourke, Brian
通讯作者：
O'Rourke, Brian
影响因子：
3.8
作者：
Biot, C;Dessolin, J;Davioud-Charvet, E
通讯作者：
Davioud-Charvet, E
影响因子：
3.8
作者：
Merrill, CL;Ni, H;Morgan, KT
通讯作者：
Morgan, KT