TRACTION FORCES GENERATED BY LOCOMOTING KERATOCYTES
TRACTION FORCES GENERATED BY LOCOMOTING KERATOCYTES
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DOI：
10.1083/jcb.127.6.1957
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发表时间：
1994-12-01
影响因子：
7.8
通讯作者：
JACOBSON, K
中科院分区：
文献类型：
--
作者：
LEE, J;LEONARD, M;JACOBSON, K
由运动的成纤维细胞产生的牵引力在柔性衬底上被观察到是扭曲的，包括薄的硅橡胶薄膜的皱折。成纤维细胞板层产生的牵引力被认为代表了细胞向前移动所需的力。然而，对于运动较快的细胞类型，如白细胞和生长锥体，无法检测到牵引力(Harris，A.K.，D.Stopak和P.Wild。1981年。自然(大斋节)。290：249-251)。我们已经开发了一种新的测试方法，其中快速移动的鱼角质细胞产生的牵引力可以通过嵌入弹性底物平面的小珠的二维位移来检测。在电池快速延伸的前缘没有检测到牵引力。相反，最大的牵引力垂直于细胞的左右边缘。角质形成细胞施加的最大牵引力约为2×10~(-8)N，牵引力模式与单个角质形成细胞的运动有关，表现为板层收缩和细胞与基质紧密接触的面积。
Traction forces produced by moving fibroblasts have been observed as distortions in flexible substrata including wrinkling of thin, silicone rubber films. Traction forces generated by fibroblast lamellae were thought to represent the forces required to move the cell forwards. However, traction forces could not be detected with faster moving cell types such as leukocytes and growth cones (Harris, A. K., D. Stopak, and P. Wild. 1981. Nature (Lend.). 290:249-251). We have developed a new assay in which traction forces produced by rapidly locomoting fish keratocytes can be detected by the two-dimensional displacements of small beads embedded in the plane of an elastic substratum. Traction forces were not detected at the rapidly extending front edge of the cell. Instead the largest traction forces were exerted perpendicular to the left and right cell margins. The maximum traction forces exerted by keratocytes were estimated to be similar to 2 X 10(-8) N. The pattern of traction forces can be related to the locomotion of a single keratocyte in terms of lamellar contractility and area of close cell-substratum contact.