Efficacy of Brazilian Propolis against Herpes Simplex Virus Type 1 Infection in Mice and Their Modes of Antiherpetic Efficacies.
Efficacy of Brazilian Propolis against Herpes Simplex Virus Type 1 Infection in Mice and Their Modes of Antiherpetic Efficacies.
复制标题
DOI：
10.1155/2011/976196
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Kurokawa M
中科院分区：
文献类型：
--
作者：
Shimizu T;Takeshita Y;Takamori Y;Kai H;Sawamura R;Yoshida H;Watanabe W;Tsutsumi A;Park YK;Yasukawa K;Matsuno K;Shiraki K;Kurokawa M
在感染后第0至6天，将巴西野牡丹的乙醇提取物（AF-06、07和08，10 mg/kg）口服给药于单纯疱疹病毒1型（HSV-1）感染的小鼠，每日三次，以评价其抗HSV-1感染的功效和显著限制疱疹性皮肤病变的发展。AF-07和08在第4天显著降低脑和/或皮肤中的病毒滴度而没有毒性，但AF-08在体外没有抗HSV-1活性。AF-06和08显著增强感染小鼠对灭活HSV-1抗原的迟发型超敏反应（DTH）。口服AF-08给药显著增加了HSV-1抗原从HSV-1感染小鼠脾细胞产生的干扰素（IFN）-γ，而感染小鼠脾细胞直接暴露于AF-06显著提高了体外IFN-γ的产生。因此，AF-08可能具有即使在口服给药后也在体内具有活性的组分，而AF-06的那些组分可能仅在体外具有活性。由于DTH是皮内HSV-1感染的主要宿主防御，因此AF-06或08分别直接或间接增强DTH应答可能有助于其对抗HSV-1感染的功效。此外，AF-06和07可能含有有助于其功效的抗HSV-1成分。这些生物学活性可用于分析其药理作用。
Ethanol extracts (AF-06, 07, and 08, 10 mg/kg) of Brazilian propolis were administered orally to cutaneously herpes simplex virus type 1 (HSV-1)-infected mice three times daily on days 0 to 6 after infection to evaluate their efficacies against HSV-1 infection and significantly limited development of herpetic skin lesions. AF-07 and 08 significantly reduced virus titers in brain and/or skin on day 4 without toxicity, but AF-08 had no anti-HSV-1 activity in vitro. AF-06 and 08 significantly enhanced delayed-type hypersensitivity (DTH) to inactivated HSV-1 antigen in infected mice. Oral AF-08-administration significantly augmented interferon (IFN)-γ production by HSV-1 antigen from splenocytes of HSV-1-infected mice, while direct exposure of splenocytes of infected mice to AF-06 significantly elevated IFN-γ production in vitro. Thus, AF-08 might have components that are active in vivo even after oral administration and those of AF-06 might be active only in vitro. Because DTH is a major host defense for intradermal HSV-1 infection, augmentation of DTH response by AF-06 or 08, directly or indirectly, respectively, may contribute to their efficacies against HSV-1 infection. In addition, AF-06 and 07 possibly contain anti-HSV-1 components contributing to their efficacies. Such biological activities of Brazilian propolis may be useful to analyze its pharmacological actions.