A gain-of-function mutation of plastidic invertase alters nuclear gene expression with sucrose treatment partially via GENOMES UNCOUPLED1-mediated signaling
A gain-of-function mutation of plastidic invertase alters nuclear gene expression with sucrose treatment partially via GENOMES UNCOUPLED1-mediated signaling
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DOI：
10.1111/nph.13309
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发表时间：
2015-05-01
期刊：
影响因子：
9.4
通讯作者：
Shigeoka, Shigeru
中科院分区：
文献类型：
--
作者：
Maruta, Takanori;Miyazaki, Nozomi;Shigeoka, Shigeru
叶绿体基因表达(PGE)是通过依赖基因组UNCOUPLED1(GUN1)的逆行信号来调节光合作用相关核基因(PhANGs)表达的信号之一。我们最近分离到了拟南芥糖诱导的子叶黄色192(SICE-192)，这是一个可塑性转化酶的功能增益型突变体，经蔗糖处理后，PhANGs和PGE的表达都降低了，这表明SICE-192突变激活了PGE诱导和GUN1介导的逆行途径。为了阐明SICE-192突变、PGE与GUN1介导的途径、叶绿体和核基因表达的关系，对GUN1缺失突变体SICE-192和GUN1-101的双突变体SICE-192和GUN-101进行了研究。蔗糖处理显著抑制了SICE-192突变体中依赖于叶绿体编码的RNA聚合酶(PEP)的PGE，但GUN1干扰并不能减轻这种抑制和子叶黄色表型。微阵列分析表明，在蔗糖处理的SICE-192突变体中，核基因如Pang的表达变化在很大程度上依赖于GUN1。目前的研究结果表明，SICE-192突变通过GUN1在蔗糖处理下改变了核基因的表达，随后可能通过抑制依赖于PEP的PGE而改变核基因的表达，这为了解叶绿体糖代谢在核基因表达中的作用提供了新的视角。
Plastid gene expression (PGE) is one of the signals that regulate the expression of photosynthesis-associated nuclear genes (PhANGs) via GENOMES UNCOUPLED1 (GUN1)-dependent retrograde signaling. We recently isolated Arabidopsis sugar-inducible cotyledon yellow-192 (sicy-192), a gain-of-function mutant of plastidic invertase, and showed that following the treatment of this mutant with sucrose, the expression of PhANGs as well as PGE decreased, suggesting that the sicy-192 mutation activates a PGE-evoked and GUN1-mediated retrograde pathway. To clarify the relationship between the sicy-192 mutation, PGE, and GUN1-mediated pathway, plastid and nuclear gene expression in a double mutant of sicy-192 and gun1-101, a null mutant of GUN1 was studied. Plastid-encoded RNA polymerase (PEP)-dependent PGE was markedly suppressed in the sicy-192 mutant by the sucrose treatment, but the suppression as well as cotyledon yellow phenotype was not mitigated by GUN1 disruption. Microarray analysis revealed that the altered expression of nuclear genes such as PhANG in the sucrose-treated sicy-192 mutant was largely dependent on GUN1. The present findings demonstrated that the sicy-192 mutation alters nuclear gene expression with sucrose treatment via GUN1, which is possibly followed by inhibiting PEP-dependent PGE, providing a new insight into the role of plastid sugar metabolism in nuclear gene expression.