Quantitative trait loci for honey bee stinging behavior and body size.
Quantitative trait loci for honey bee stinging behavior and body size.
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发表时间：
1998-03
期刊：
影响因子：
3.3
通讯作者：
G. Hunt;E. Guzman‐Novoa;M. Fondrk;R. Page
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文献类型：
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作者：
G. Hunt;E. Guzman‐Novoa;M. Fondrk;R. Page
对影响蜜蜂群体水平蜇刺行为和个体大小的数量性状基因座（QTL）进行了研究。一只F1蜂王是由一只欧洲起源的蜂王和一只非洲亚种的雄蜂杂交产生的。将来自杂交蜂后的单倍体雄蜂与姐妹欧洲蜂后单独回交以产生172个具有回交工蜂的菌落，所述回交工蜂被评价为刺的倾向。随机扩增的多态性DNA标记进行了评分，从这些殖民地的单倍体雄蜂父亲。工蜂和雄蜂的翅膀被用来衡量体型，因为美洲的非洲化蜜蜂比欧洲蜜蜂小。采用标准区间作图和多QTL模型对数据进行分析。一个可能的QTL被确定为具有显著影响的倾向刺痛（LOD 3.57）。还观察到4个其它的暗示性QTL（约LOD 1.5）。还确定了可能的QTL，影响身体的大小，并与防御行为的QTL。其中两个具有显著性（LOD 3.54和5.15）。
A study was conducted to identify quantitative trait loci (QTLs) that affect colony-level stinging behavior and individual body size of honey bees. An F1 queen was produced from a cross between a queen of European origin and a drone descended from an African subspecies. Haploid drones from the hybrid queen were individually backcrossed to sister European queens to produce 172 colonies with backcross workers that were evaluated for tendency to sting. Random amplified polymorphic DNA markers were scored from the haploid drone fathers of these colonies. Wings of workers and drones were used as a measure of body size because Africanized bees in the Americas are smaller than European bees. Standard interval mapping and multiple QTL models were used to analyze data. One possible QTL was identified with a significant effect on tendency to sting (LOD 3.57). Four other suggestive QTLs were also observed (about LOD 1.5). Possible QTLs also were identified that affect body size and were unlinked to defensive-behavior QTLs. Two of these were significant (LOD 3.54 and 5.15).